Lysophosphatidic acid-induced calcium signals in cultured rat oligodendrocytes
Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review
Author(s)
Detail(s)
Original language | English |
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Pages (from-to) | 2929-2932 |
Journal / Publication | NeuroReport |
Volume | 10 |
Issue number | 14 |
Publication status | Published - 29 Sept 1999 |
Externally published | Yes |
Link(s)
Abstract
OLIGODENDROCYTES are the myelin forming glial cells of the CNS and are known to express receptors linked to ion channels and intracellular second messenger cascades. In this paper, we describe the intracellular calcium responses of cells from the oligodendrocyte lineage to application of lysophosphatidic acid (LPA), a naturally occurring, growth factor-like phospholipid. Oligodendrocyte precursors did not respond to application of LPA (1 μM). In mature oligodendrocytes, however, LPA (1 μM) induced an increase in the intracellular calcium concentration ([Ca2+]i). In the majority of cells this increase was followed by a persistent plateau phase. The LPA-induced [Ca2+]i signal vanished in Ca2+-free medium, implying that it arose due to a Ca2+ influx across the plasma membrane. Preincubation of the cells with Pertussis-toxin prevented the generation of LPA-induced [Ca2+]i signals. We conclude that cultured rat oligodendrocytes express functional LPA receptors, which mediate a transmembrane Ca2+ influx via a Pertussis-toxin-sensitive G-protein.
Research Area(s)
- Calcium imaging, Lysophosphatidic acid, Oligodendrocytes
Citation Format(s)
Lysophosphatidic acid-induced calcium signals in cultured rat oligodendrocytes. / Möller, Thomas; Musante, David B.; Ransom, Bruce R.
In: NeuroReport, Vol. 10, No. 14, 29.09.1999, p. 2929-2932.
In: NeuroReport, Vol. 10, No. 14, 29.09.1999, p. 2929-2932.
Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review