Luminescent ruthenium(II) amidodipyridoquinoxaline biotin complexes that display higher avidin-induced emission enhancement
Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review
Author(s)
Detail(s)
Original language | English |
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Pages (from-to) | 293-302 |
Journal / Publication | Inorganica Chimica Acta |
Volume | 360 |
Issue number | 1 |
Publication status | Published - 1 Jan 2007 |
Link(s)
Abstract
A series of luminescent ruthenium(II) amidodipyridoquinoxaline biotin (dpq-B) complexes [Ru(N-N)2(N-N′)](PF6)2 (N-N = 2,2′-bipyridine (bpy), 1,10-phenanthroline (phen), 4,7-diphenyl-1,10-phenanthroline (Ph2-phen); N-N′ = 2-((2-biotinamido)ethyl)amidodipyrido[3,2-f:2′,3′-h]quinoxaline (dpq-C2-B), 2-((6-biotinamido)hexyl)amidodipyrido[3,2-f:2′,3′-h]quinoxaline (dpq-C6-B)) has been designed as new luminescent probes for avidin. The electrochemical and photophysical properties of these complexes have been investigated. Upon irradiation, all the complexes exhibited metal-to-ligand charge-transfer (3MLCT) (dπ(Ru) → π*(diimine)) emission in fluid solutions at 298 K and in low-temperature glass. In aqueous buffer, the emission was extremely weak, probably a consequence of hydrogen-bonding interactions between the amide moiety of the dpq-B ligands and the water molecules. The avidin-binding properties of all the complexes have been studied by 4′-hydroxyazobenzene-2-carboxylic acid (HABA) assays, luminescence titrations, kinetics experiments and confocal microscopy using avidin-conjugated microspheres. © 2006 Elsevier B.V. All rights reserved.
Research Area(s)
- Avidin, Biotin, Luminescence, Probes, Ruthenium complexes
Citation Format(s)
Luminescent ruthenium(II) amidodipyridoquinoxaline biotin complexes that display higher avidin-induced emission enhancement. / Lo, Kenneth Kam-Wing; Lee, Terence Kwok-Ming.
In: Inorganica Chimica Acta, Vol. 360, No. 1, 01.01.2007, p. 293-302.
In: Inorganica Chimica Acta, Vol. 360, No. 1, 01.01.2007, p. 293-302.
Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review