Luminescent rhenium(I) polypyridine fluorous complexes as new biological probes

Man-Wai Louie; Tommy Tsz-Him Fong; Kenneth Kam-Wing Lo

doi:10.1109/NANOMED.2010.5749806

Luminescent rhenium(I) polypyridine fluorous complexes as new biological probes

Man-Wai Louie, Tommy Tsz-Him Fong, Kenneth Kam-Wing Lo^*

^*Corresponding author for this work

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

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Abstract

We present the synthesis and characterization of luminescent biological probes derived from rhenium(I) polypyridine fluorous complexes [Re(Me ₂ bpy)(CO)₃(py-Rf-R)](PF₆) (R = NH₂ (1), NCS (2), TU-C₂H₅ (3)). The photophysical properties of these complexes have been studied. The fluorous complex 2 has been used to label glutathione (GSH) and bovine serum albumin (BSA). The photophysical properties of the resultant bioconjugates have been studied. The isolation of the luminescent fluorous rhenium-GSH conjugate from a mixture of twenty amino acids has been demonstrated using fluorous solidphase extraction (FSPE). Additionally, the cytotoxicity of complexes 1 and 3 toward HeLa cells has been examined by the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide (MTT) assay. The cellular uptake properties of complex 3 have been investigated by laser-scanning confocal microscopy. © 2010 IEEE.

Original language	English
Title of host publication	2010 IEEE International Conference on Nano/Molecular Medicine and Engineering, IEEE NANOMED 2010
Pages	60-65
DOIs	https://doi.org/10.1109/NANOMED.2010.5749806
Publication status	Published - 2010
Event	4th IEEE International Conference on Nano/Molecular Medicine and Engineering (IEEE NANOMED 2010) - Hong Kong/Macau, China Duration: 5 Dec 2010 → 9 Dec 2010

Conference

Conference	4th IEEE International Conference on Nano/Molecular Medicine and Engineering (IEEE NANOMED 2010)
Country/Territory	China
City	Hong Kong/Macau
Period	5/12/10 → 9/12/10

Research Keywords

fluorous
labeling
luminescence
proteins
rhenium

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title = "Luminescent rhenium(I) polypyridine fluorous complexes as new biological probes",

abstract = "We present the synthesis and characterization of luminescent biological probes derived from rhenium(I) polypyridine fluorous complexes [Re(Me 2 bpy)(CO)3(py-Rf-R)](PF6) (R = NH2 (1), NCS (2), TU-C2H5 (3)). The photophysical properties of these complexes have been studied. The fluorous complex 2 has been used to label glutathione (GSH) and bovine serum albumin (BSA). The photophysical properties of the resultant bioconjugates have been studied. The isolation of the luminescent fluorous rhenium-GSH conjugate from a mixture of twenty amino acids has been demonstrated using fluorous solidphase extraction (FSPE). Additionally, the cytotoxicity of complexes 1 and 3 toward HeLa cells has been examined by the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide (MTT) assay. The cellular uptake properties of complex 3 have been investigated by laser-scanning confocal microscopy. {\textcopyright} 2010 IEEE.",

keywords = "fluorous, labeling, luminescence, proteins, rhenium",

author = "Man-Wai Louie and Fong, {Tommy Tsz-Him} and Lo, {Kenneth Kam-Wing}",

year = "2010",

doi = "10.1109/NANOMED.2010.5749806",

language = "English",

isbn = "9781612841533",

pages = "60--65",

booktitle = "2010 IEEE International Conference on Nano/Molecular Medicine and Engineering, IEEE NANOMED 2010",

note = "4th IEEE International Conference on Nano/Molecular Medicine and Engineering (IEEE NANOMED 2010) ; Conference date: 05-12-2010 Through 09-12-2010",

}

Louie, M-W, Fong, TT-H & Lo, KK-W 2010, Luminescent rhenium(I) polypyridine fluorous complexes as new biological probes. in 2010 IEEE International Conference on Nano/Molecular Medicine and Engineering, IEEE NANOMED 2010., 5749806, pp. 60-65, 4th IEEE International Conference on Nano/Molecular Medicine and Engineering (IEEE NANOMED 2010), Hong Kong/Macau, China, 5/12/10. https://doi.org/10.1109/NANOMED.2010.5749806

Luminescent rhenium(I) polypyridine fluorous complexes as new biological probes. / Louie, Man-Wai; Fong, Tommy Tsz-Him; Lo, Kenneth Kam-Wing.
2010 IEEE International Conference on Nano/Molecular Medicine and Engineering, IEEE NANOMED 2010. 2010. p. 60-65 5749806.

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

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T1 - Luminescent rhenium(I) polypyridine fluorous complexes as new biological probes

AU - Louie, Man-Wai

AU - Fong, Tommy Tsz-Him

AU - Lo, Kenneth Kam-Wing

PY - 2010

Y1 - 2010

N2 - We present the synthesis and characterization of luminescent biological probes derived from rhenium(I) polypyridine fluorous complexes [Re(Me 2 bpy)(CO)3(py-Rf-R)](PF6) (R = NH2 (1), NCS (2), TU-C2H5 (3)). The photophysical properties of these complexes have been studied. The fluorous complex 2 has been used to label glutathione (GSH) and bovine serum albumin (BSA). The photophysical properties of the resultant bioconjugates have been studied. The isolation of the luminescent fluorous rhenium-GSH conjugate from a mixture of twenty amino acids has been demonstrated using fluorous solidphase extraction (FSPE). Additionally, the cytotoxicity of complexes 1 and 3 toward HeLa cells has been examined by the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide (MTT) assay. The cellular uptake properties of complex 3 have been investigated by laser-scanning confocal microscopy. © 2010 IEEE.

AB - We present the synthesis and characterization of luminescent biological probes derived from rhenium(I) polypyridine fluorous complexes [Re(Me 2 bpy)(CO)3(py-Rf-R)](PF6) (R = NH2 (1), NCS (2), TU-C2H5 (3)). The photophysical properties of these complexes have been studied. The fluorous complex 2 has been used to label glutathione (GSH) and bovine serum albumin (BSA). The photophysical properties of the resultant bioconjugates have been studied. The isolation of the luminescent fluorous rhenium-GSH conjugate from a mixture of twenty amino acids has been demonstrated using fluorous solidphase extraction (FSPE). Additionally, the cytotoxicity of complexes 1 and 3 toward HeLa cells has been examined by the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide (MTT) assay. The cellular uptake properties of complex 3 have been investigated by laser-scanning confocal microscopy. © 2010 IEEE.

KW - fluorous

KW - labeling

KW - luminescence

KW - proteins

KW - rhenium

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U2 - 10.1109/NANOMED.2010.5749806

DO - 10.1109/NANOMED.2010.5749806

M3 - RGC 32 - Refereed conference paper (with host publication)

SN - 9781612841533

SP - 60

EP - 65

BT - 2010 IEEE International Conference on Nano/Molecular Medicine and Engineering, IEEE NANOMED 2010

T2 - 4th IEEE International Conference on Nano/Molecular Medicine and Engineering (IEEE NANOMED 2010)

Y2 - 5 December 2010 through 9 December 2010

ER -

Luminescent rhenium(I) polypyridine fluorous complexes as new biological probes

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