α-L-Threose Nucleic Acids as Biocompatible Antisense Oligonucleotides for Suppressing Gene Expression in Living Cells

Research output: Conference Papers (RGC: 31A, 31B, 32, 33)Poster

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Detail(s)

Original languageEnglish
Publication statusPublished - Jun 2018

Workshop

Title3rd Functional DNA Nanotechnology Workshop
LocationPalazzetto Mattei
PlaceItaly
CityRome
Period6 - 8 June 2018

Abstract

We investigated the biological properties and examined the potency of α-L-Threose nucleic acid (TNA) polymers to suppress gene expression in living cells. We discovered that the TNA polymers exhibit strong affinity and specificity towards the complementary DNA and RNA targets, are highly biocompatible and non-toxic in living cell system, and readily enter a number of cell lines without using transfecting agents. Particularly, TNA exhibit better stability toward fetal bovine serum or human serum as compared to traditional antisense oligonucleotide which mean that the intrinsic structure of TNA is thoroughly resistant to biological degradation. Importantly, the efficacy of TNA polymer with GFP target sequence as antisense agents was firstly demonstrated in cellular conditions in which these polymers revealed high antisense activity in terms of the degree of inhibition of GFP gene expression. The GFP inhibition studies testified the TNA as a functional biomaterial and a valuable alternative to traditional antisense oligonucleotide such as PNAs, MOs and LNAs for a wide range of applications in drug discovery and life science research.

Citation Format(s)

α-L-Threose Nucleic Acids as Biocompatible Antisense Oligonucleotides for Suppressing Gene Expression in Living Cells. / Liu, Ling Sum; Leung, Hoi Man; Tam, Dick Yan; Lo, Tsz Wan; Wong, Sze Wing ; Lo, Pik Kwan .

2018. Poster session presented at 3rd Functional DNA Nanotechnology Workshop, Rome, Italy.

Research output: Conference Papers (RGC: 31A, 31B, 32, 33)Poster