Low-Toxicity Sulfonium-Based Probes for Cysteine-Specific Profiling in Live Cells

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

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Author(s)

  • Rui Wang
  • Dongyan Yang
  • Yuhao An
  • Chuan Wan
  • Qi Chang
  • Mingchan Liang
  • Zhanfeng Hou
  • Zigang Li

Detail(s)

Original languageEnglish
Pages (from-to)4366-4372
Journal / PublicationAnalytical Chemistry
Volume94
Issue number10
Online published4 Mar 2022
Publication statusPublished - 15 Mar 2022

Abstract

Despite being a low-abundance amino acid, cysteine plays an essential role in regulating protein function and serves as a satisfactory target of post-translational modifications and drug developments. To comprehensively assess reactive-cysteine-containing proteins, the development of chemical proteomic probes to label cysteine residues in human cells is an important objective. Cysteine modification using sulfonium-based probes is a novel method to identify reactive cysteine residues in proteins. Herein, we reported a set of "cysteine-reactive sulfonium-based (C-Sul)" probes to label the reactive cysteine sites in cellular proteins. Notably, water-soluble C-Sul probes have a significantly enhanced stability and cellular uptakes, displaying a high specificity toward reactive cysteines and compatibility with quantitative proteomic profiling. In comparison to the conventional iodoacetamide-based probe, C-Sul particularly has no inhibitory effects on cell viability, enabling its application in proteomic profiling of reactive cysteine residues under biorelevant conditions. We propose C-Sul probes as optimal tools of cysteine profiling for further broadly basic research. © 2022 American Chemical Society.

Citation Format(s)

Low-Toxicity Sulfonium-Based Probes for Cysteine-Specific Profiling in Live Cells. / Wang, Rui; Yang, Dongyan; Tian, Tian et al.
In: Analytical Chemistry, Vol. 94, No. 10, 15.03.2022, p. 4366-4372.

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review