Loss of Astrocytic Domain Organization in the Epileptic Brain

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Nancy Ann Oberheim
  • Guo-Feng Tian
  • Xiaoning Han
  • Weiguo Peng
  • Takahiro Takano
  • Maiken Nedergaard

Detail(s)

Original languageEnglish
Pages (from-to)3264-3276
Journal / PublicationJournal of Neuroscience
Volume28
Issue number13
Publication statusPublished - 26 Mar 2008
Externally publishedYes

Abstract

Gliosis is a pathological hallmark of posttraumatic epileptic foci, but little is known about these reactive astrocytes beyond their high glial fibrillary acidic protein (GFAP) expression. Using diolistic labeling, we show that cortical astrocytes lost their nonoverlapping domain organization in three mouse models of epilepsy: posttraumatic injury, genetic susceptibility, and systemic kainate exposure. Neighboring astrocytes in epileptic mice showed a 10-fold increase in overlap of processes. Concurrently, spine density was increased on dendrites of excitatory neurons. Suppression of seizures by the common antiepileptic, valproate, reduced the overlap of astrocytic processes. Astrocytic domain organization was also preserved in APP transgenic mice expressing a mutant variant of human amyloid precursor protein despite a marked upregulation of GFAP. Our data suggest that loss of astrocytic domains was not universally associated with gliosis, but restricted to seizure pathologies. Reorganization of astrocytes may, in concert with dendritic sprouting and new synapse formation, form the structural basis for recurrent excitation in the epileptic brain. Copyright © 2008 Society for Neuroscience.

Research Area(s)

  • Dendritic sprouting, Diolistic labeling, EEG, Fluorescence microscopy, Glia, Mice model

Citation Format(s)

Loss of Astrocytic Domain Organization in the Epileptic Brain. / Oberheim, Nancy Ann; Tian, Guo-Feng; Han, Xiaoning; Peng, Weiguo; Takano, Takahiro; Ransom, Bruce; Nedergaard, Maiken.

In: Journal of Neuroscience, Vol. 28, No. 13, 26.03.2008, p. 3264-3276.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review