Long-lived protein expression in hydrogel particles : Towards artificial cells

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

41 Scopus Citations
View graph of relations

Author(s)

Detail(s)

Original languageEnglish
Pages (from-to)4275-4279
Journal / PublicationChemical Science
Volume9
Issue number18
Online published16 Apr 2018
Publication statusPublished - 14 May 2018
Externally publishedYes

Link(s)

Abstract

Herein we report a new type of cell-mimic particle capable of long-lived protein expression. We constructed the cell-mimic particles by immobilizing the proteinaceous factors of the cell-free transcription and translation system on the polymer backbone of hydrogel particles and encapsulating the plasmid template and ribosome inside the hydrogel. With the continuous supply of nutrients and energy, the protein expression in the cell-mimic particles remained stable for at least 11 days. We achieved the regulation of protein expression in the cell-mimic particles by the usage of lac operon. The cell-mimic particles quickly responded to the concentration change of isopropyl β-D-1-thiogalactopyranoside (IPTG) in the feeding buffer to regulate the mCherry expression level. We also constructed an in vitro genetic oscillator in the cell-mimic particles. Protein LacI provided a negative feedback to the expression of both LacI itself and eGFP, and the expression level change of eGFP presented an oscillation. We expect the cell-mimic particles to be a useful platform for gene circuit engineering, metabolic engineering, and biosensors. © The Royal Society of Chemistry 2018.

Research Area(s)

Citation Format(s)

Long-lived protein expression in hydrogel particles: Towards artificial cells. / Zhou, Xiaoyu; Wu, Han; Cui, Miao et al.
In: Chemical Science, Vol. 9, No. 18, 14.05.2018, p. 4275-4279.

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

Download Statistics

No data available