Long-term storage of aerobic granules in liquid media: Viable but non-culturable status

Chunli Wan; Qinlan Zhang; Duu-Jong Lee; Yayi Wang; Jieni Li

doi:10.1016/j.biortech.2014.05.091

Long-term storage of aerobic granules in liquid media: Viable but non-culturable status

Chunli Wan, Qinlan Zhang, Duu-Jong Lee, Yayi Wang, Jieni Li

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

42 Citations (Scopus)

Abstract

Long-term storage and successful reactivation after storage are essential for practical applications of aerobic granules on wastewater treatment. This study cultivated aerobic granules (SI) in sequencing batch reactors and then stored the granules at 4. °C in five liquid media (DI water (SW), acetone (SA), acetone/isoamyl acetate mix (SAA), saline water (SS), and formaldehyde (SF)) for over 1. year. The first four granules were then successfully reactivated in 24. h cultivation. The specific oxygen uptake rates (SOUR) of the granules followed SI. >. SS. >. SA. >. SAA. >. SW. >. SF; and the corresponding granular strengths (10. min ultrasound) followed SI. >. SA = SS. >. SAA. >. SW. >>. SF. During storage the granular cells secreted excess quantities of cyclic-diguanylate (c-di-GMP) and pentaphosphate (ppGpp) as responses to the stringent challenges. We proposed that to force cells in granules (Alphaproteobacteria, Flavobacteria, Betaproteobacteria, Gammaproteobacteria, Actinobacteria, Sphingobacteria, and Clostridia) entering viable but non-culturable (VBNC) status is the key of success for extended period storage of granules. © 2014 Elsevier Ltd.

Original language	English
Pages (from-to)	464-470
Journal	Bioresource Technology
Volume	166
DOIs	https://doi.org/10.1016/j.biortech.2014.05.091
Publication status	Published - Aug 2014
Externally published	Yes

Bibliographical note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 6 Clean Water and Sanitation

Research Keywords

Aerobic granules
High throughput sequencing
Signaling molecules
Storage
Viable but non-culturable status

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10.1016/j.biortech.2014.05.091

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title = "Long-term storage of aerobic granules in liquid media: Viable but non-culturable status",

abstract = "Long-term storage and successful reactivation after storage are essential for practical applications of aerobic granules on wastewater treatment. This study cultivated aerobic granules (SI) in sequencing batch reactors and then stored the granules at 4. °C in five liquid media (DI water (SW), acetone (SA), acetone/isoamyl acetate mix (SAA), saline water (SS), and formaldehyde (SF)) for over 1. year. The first four granules were then successfully reactivated in 24. h cultivation. The specific oxygen uptake rates (SOUR) of the granules followed SI. >. SS. >. SA. >. SAA. >. SW. >. SF; and the corresponding granular strengths (10. min ultrasound) followed SI. >. SA = SS. >. SAA. >. SW. >>. SF. During storage the granular cells secreted excess quantities of cyclic-diguanylate (c-di-GMP) and pentaphosphate (ppGpp) as responses to the stringent challenges. We proposed that to force cells in granules (Alphaproteobacteria, Flavobacteria, Betaproteobacteria, Gammaproteobacteria, Actinobacteria, Sphingobacteria, and Clostridia) entering viable but non-culturable (VBNC) status is the key of success for extended period storage of granules. {\textcopyright} 2014 Elsevier Ltd.",

keywords = "Aerobic granules, High throughput sequencing, Signaling molecules, Storage, Viable but non-culturable status",

author = "Chunli Wan and Qinlan Zhang and Duu-Jong Lee and Yayi Wang and Jieni Li",

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doi = "10.1016/j.biortech.2014.05.091",

language = "English",

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journal = "Bioresource Technology",

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TY - JOUR

T1 - Long-term storage of aerobic granules in liquid media

T2 - Viable but non-culturable status

AU - Wan, Chunli

AU - Zhang, Qinlan

AU - Lee, Duu-Jong

AU - Wang, Yayi

AU - Li, Jieni

N1 - Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

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N2 - Long-term storage and successful reactivation after storage are essential for practical applications of aerobic granules on wastewater treatment. This study cultivated aerobic granules (SI) in sequencing batch reactors and then stored the granules at 4. °C in five liquid media (DI water (SW), acetone (SA), acetone/isoamyl acetate mix (SAA), saline water (SS), and formaldehyde (SF)) for over 1. year. The first four granules were then successfully reactivated in 24. h cultivation. The specific oxygen uptake rates (SOUR) of the granules followed SI. >. SS. >. SA. >. SAA. >. SW. >. SF; and the corresponding granular strengths (10. min ultrasound) followed SI. >. SA = SS. >. SAA. >. SW. >>. SF. During storage the granular cells secreted excess quantities of cyclic-diguanylate (c-di-GMP) and pentaphosphate (ppGpp) as responses to the stringent challenges. We proposed that to force cells in granules (Alphaproteobacteria, Flavobacteria, Betaproteobacteria, Gammaproteobacteria, Actinobacteria, Sphingobacteria, and Clostridia) entering viable but non-culturable (VBNC) status is the key of success for extended period storage of granules. © 2014 Elsevier Ltd.

AB - Long-term storage and successful reactivation after storage are essential for practical applications of aerobic granules on wastewater treatment. This study cultivated aerobic granules (SI) in sequencing batch reactors and then stored the granules at 4. °C in five liquid media (DI water (SW), acetone (SA), acetone/isoamyl acetate mix (SAA), saline water (SS), and formaldehyde (SF)) for over 1. year. The first four granules were then successfully reactivated in 24. h cultivation. The specific oxygen uptake rates (SOUR) of the granules followed SI. >. SS. >. SA. >. SAA. >. SW. >. SF; and the corresponding granular strengths (10. min ultrasound) followed SI. >. SA = SS. >. SAA. >. SW. >>. SF. During storage the granular cells secreted excess quantities of cyclic-diguanylate (c-di-GMP) and pentaphosphate (ppGpp) as responses to the stringent challenges. We proposed that to force cells in granules (Alphaproteobacteria, Flavobacteria, Betaproteobacteria, Gammaproteobacteria, Actinobacteria, Sphingobacteria, and Clostridia) entering viable but non-culturable (VBNC) status is the key of success for extended period storage of granules. © 2014 Elsevier Ltd.

KW - Aerobic granules

KW - High throughput sequencing

KW - Signaling molecules

KW - Storage

KW - Viable but non-culturable status

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DO - 10.1016/j.biortech.2014.05.091

M3 - RGC 21 - Publication in refereed journal

C2 - 24950091

SN - 0960-8524

VL - 166

SP - 464

EP - 470

JO - Bioresource Technology

JF - Bioresource Technology

ER -

Long-term storage of aerobic granules in liquid media: Viable but non-culturable status

Abstract

Bibliographical note

UN SDGs

Research Keywords

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