Long Non-coding RNA RUNDC3A-AS1 Promotes Lung Metastasis of Thyroid Cancer via Targeting the miR-182-5p/ADAM9

Dawei Ma; Yan Zhu; Xiao Zhang; Jia Zhang; Wei Chen; Xinyuan Chen; Yichun Qian; Yanbin Zhao; Tingting Hu; Zhangyu Yao; Wei Zhao; Yuan Zhang; Fangzhou Liu

doi:10.3389/fcell.2021.650004

Author(s)

Dawei Ma
Yan Zhu
Xiao Zhang
Jia Zhang
Wei Chen
And 8 others

Xinyuan Chen
Yichun Qian
Yanbin Zhao
Tingting Hu
Zhangyu Yao
Wei Zhao
Yuan Zhang
Fangzhou Liu

Detail(s)

Original language	English
Article number	650004
Journal / Publication	Frontiers in Cell and Developmental Biology
Volume	9
Online published	11 May 2021
Publication status	Published - May 2021
Externally published	Yes

Link(s)

DOI	DOI https://doi.org/10.3389/fcell.2021.650004 Final Published version
	Check@CityULib
Attachment(s)	Documents 84804457.pdf Final Published version, 16.8 MB, PDF Publisher's Copyright Statement This full text is made available under CC-BY 4.0. https://creativecommons.org/licenses/by/4.0/
Link to Scopus	https://www.scopus.com/record/display.uri?eid=2-s2.0-85107067472&origin=recordpage
Permanent Link	https://scholars.cityu.edu.hk/en/publications/publication(803bd571-086b-4488-867d-dd6543d7ddc6).html

Abstract

Long non-coding RNAs (lncRNAs) have been identified as influential indicators in variety of malignancies. Among which, LncRNA RUNDC3A-AS1 is reported to upregulate in thyroid cancer. However, the expression pattern and the pathological function of lncRNA RUNDC3A-AS1 in thyroid cancer is unclear. In this study, we examined the expression levels of lncRNA RUNDC3A-AS1 in the thyroid cancer tissues and cell lines via RT-qPCR analysis. The effects of RUNDC3A-AS1 on thyroid cancer cell metastasis were detected by transwell chamber assay, scratch assay in vitro and lung metastasis model in vivo. The results indicated that RUNDC3A-AS1 was highly expressed in the thyroid cancer tissues and cell lines. Functionally, knockdown of RUNDC3A-AS1 could repress the migration and invasion of thyroid cancer cells in vitro, and inhibit thyroid cancer metastasis to lung in vivo. Mechanistically, RUNDC3A-AS1 served as an inhibitor of miR-182-5p in tumor tissues and cell lines. RUNDC3A-AS1 inhibited the expression of miR-182-5p to increase the expression level of ADAM9, thus further aggravating the malignancy of thyroid cancer. Therefore, the RUNDC3A-AS1/miR-182-5p/ADAM9 axis may be a potential therapeutic target for the treatment of thyroid cancer metastasis.

Research Area(s)

in vivo mice model, lncRNA RUNDC3A-AS1, metastasis, miR-182-5p/ADAM9, thyroid cancer

SDG 3 - Good Health and Well-being

Citation Format(s)

[ RIS ] [ BibTeX ]

Long Non-coding RNA RUNDC3A-AS1 Promotes Lung Metastasis of Thyroid Cancer via Targeting the miR-182-5p/ADAM9. / Ma, Dawei; Zhu, Yan; Zhang, Xiao et al.
In: Frontiers in Cell and Developmental Biology, Vol. 9, 650004, 05.2021.

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

Ma, D, Zhu, Y, Zhang, X, Zhang, J, Chen, W, Chen, X, Qian, Y, Zhao, Y, Hu, T, Yao, Z, Zhao, W, Zhang, Y & Liu, F 2021, 'Long Non-coding RNA RUNDC3A-AS1 Promotes Lung Metastasis of Thyroid Cancer via Targeting the miR-182-5p/ADAM9', Frontiers in Cell and Developmental Biology, vol. 9, 650004. https://doi.org/10.3389/fcell.2021.650004

Ma, D., Zhu, Y., Zhang, X., Zhang, J., Chen, W., Chen, X., Qian, Y., Zhao, Y., Hu, T., Yao, Z., Zhao, W., Zhang, Y., & Liu, F. (2021). Long Non-coding RNA RUNDC3A-AS1 Promotes Lung Metastasis of Thyroid Cancer via Targeting the miR-182-5p/ADAM9. Frontiers in Cell and Developmental Biology, 9, Article 650004. https://doi.org/10.3389/fcell.2021.650004

Ma D, Zhu Y, Zhang X, Zhang J, Chen W, Chen X et al. Long Non-coding RNA RUNDC3A-AS1 Promotes Lung Metastasis of Thyroid Cancer via Targeting the miR-182-5p/ADAM9. Frontiers in Cell and Developmental Biology. 2021 May;9:650004. Epub 2021 May 11. doi: 10.3389/fcell.2021.650004

Ma, Dawei ; Zhu, Yan ; Zhang, Xiao et al. / Long Non-coding RNA RUNDC3A-AS1 Promotes Lung Metastasis of Thyroid Cancer via Targeting the miR-182-5p/ADAM9. In: Frontiers in Cell and Developmental Biology. 2021 ; Vol. 9.

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