Lockd promotes myoblast proliferation and muscle regeneration via binding with DHX36 to facilitate 5' UTR rG4 unwinding and Anp32e translation

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

6 Scopus Citations
View graph of relations

Author(s)

  • Xiaona Chen
  • Guang Xue
  • Yuwei Zhang
  • Suyang Zhang
  • Wen Wang
  • Yang Li
  • Jie Yuan
  • Liangqiang He
  • Chun Yin Chan
  • Yan Liu
  • Wei Chen
  • Yu Zhao
  • Ping Hu
  • Hao Sun
  • Huating Wang

Detail(s)

Original languageEnglish
Article number110907
Journal / PublicationCell Reports
Volume39
Issue number10
Online published7 Jun 2022
Publication statusPublished - 7 Jun 2022

Link(s)

Abstract

Adult muscle stem cells, also known as satellite cells (SCs), play pivotal roles in muscle regeneration, and long non-coding RNA (lncRNA) functions in SCs remain largely unknown. Here, we identify a lncRNA, Lockd, which is induced in activated SCs upon acute muscle injury. We demonstrate that Lockd promotes SC proliferation; deletion of Lockd leads to cell-cycle arrest, and in vivo repression of Lockd in mouse muscles hinders regeneration process. Mechanistically, we show that Lockd directly interacts with RNA helicase DHX36 and the 5′end of Lockd possesses the strongest binding with DHX36. Furthermore, we demonstrate that Lockd stabilizes the interaction between DHX36 and EIF3B proteins; synergistically, this complex unwinds the RNA G-quadruplex (rG4) structure formed at Anp32e mRNA 5′ UTR and promotes the translation of ANP32E protein, which is required for myoblast proliferation. Altogether, our findings identify a regulatory Lockd/DHX36/Anp32e axis that promotes myoblast proliferation and acute-injury-induced muscle regeneration.

Research Area(s)

  • ANP32E, CP: Molecular biology, CP: Stem cell research, DHX36, EIF3B, Lockd, muscle regeneration, post-transcriptional regulation, RNA G-quadruplexes, satellite cells, translational regulation

Citation Format(s)

Lockd promotes myoblast proliferation and muscle regeneration via binding with DHX36 to facilitate 5' UTR rG4 unwinding and Anp32e translation. / Chen, Xiaona; Xue, Guang; Zhao, Jieyu et al.
In: Cell Reports, Vol. 39, No. 10, 110907, 07.06.2022.

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

Download Statistics

No data available