LncRNA CRNDE regulates the proliferation and migration of vascular smooth muscle cells

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

17 Scopus Citations
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Author(s)

  • Yu Zhou
  • Xuyu He
  • Ruiming Liu
  • Yuansen Qin
  • Shenming Wang
  • Chunying Li
  • Zuojun Hu

Detail(s)

Original languageEnglish
Pages (from-to)16205-16214
Journal / PublicationJournal of Cellular Physiology
Volume234
Issue number9
Online published10 Feb 2019
Publication statusPublished - Sept 2019

Abstract

Restenosis after angioplasty or stent is a major clinical problem. While long noncoding RNAs (lncRNAs) are implicated in a variety of diseases, their role in restenosis is not well understood. This study aims to investigate how dysregulated lncRNAs and messenger RNAs (mRNAs) contribute to restenosis. By microarray analysis, we identified 202 lncRNAs and 625 mRNAs (fold change > 2.0, p < 0.05) differentially expressed between the balloon-injured carotid artery and uninjured carotid artery in the rats. Among differentially expressed lncRNAs, LncRNA CRNDE had the highest fold change and the change was validated by reverse transcription polymerase chain reaction. We found that LncRNA CRNDE was significantly upregulated in injured rat carotid artery and vascular smooth muscle cells (VSMCs) stimulated by platelet-derived growth factor-BB (PDGF-BB). Knockdown of LncRNA CRNDE by small interference RNA significantly inhibited PDGF-BB stimulated proliferation and migration of VSMCs. Moreover, knockdown of LncRNA CRNDE attenuated PDGF-BB-induced phenotypic change of VSMCs. Taken together, our study reveals a novel mechanoresponsive LncRNA CRNDE which may be a therapeutic target for restenosis.

Research Area(s)

  • LncRNA CRNDE, microarray, migration, proliferation, vascular smooth muscle cell

Citation Format(s)

LncRNA CRNDE regulates the proliferation and migration of vascular smooth muscle cells. / Zhou, Yu; He, Xuyu; Liu, Ruiming et al.
In: Journal of Cellular Physiology, Vol. 234, No. 9, 09.2019, p. 16205-16214.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review