@inbook{41ad4bc691e849f6ba1d71188856e286,
title = "Live Imaging of Planaria",
abstract = "Planarian regeneration involves a complex series of cellular events, precisely choreographed in space and time. Time-lapse imaging can provide powerful insights into tissue dynamics, as variously demonstrated in other model systems. However, time-lapse imaging of planarians has proven to be a challenge. Especially the requisite immobilization of the animals over extended periods of time is difficult, owing to their photophobic behavior and soft body architecture. Here, we describe a new embedding method using 2\% (w/v) low melting agarose, and demonstrate that this method can effectively immobilize animals as long as 7 days. In combination with cell-permeable fluorescent dyes, this immobilization method allows for the time-lapse imaging of planaria during regeneration and other physiological processes.",
keywords = "Fluorescent dye labeling, Live imaging, Planarian, Regeneration, Time-lapse microscopy",
author = "Wei Shen and Yun Shen and Lam, \{Yun Wah\} and Danny Chan",
year = "2018",
doi = "10.1007/978-1-4939-7802-1\_22",
language = "English",
isbn = "978-1-4939-7800-7",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "507--518",
editor = "Rink, \{Jochen C.\}",
booktitle = "Planarian Regeneration",
address = "United States",
}