Liquid biopsy and therapeutic response : Circulating tumor cell cultures for evaluation of anticancer treatment

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Gianluca Grenci
  • Tengyang Jing
  • Ying Bena Lim
  • Soo Chin Lee
  • Jean Paul Thiery
  • Jongyoon Han
  • Chwee Teck Lim

Detail(s)

Original languageEnglish
Article numbere1600274
Journal / PublicationScience Advances
Volume2
Issue number7
Online published13 Jul 2016
Publication statusPublished - Jul 2016
Externally publishedYes

Link(s)

Abstract

The lack of a robust anticancer drug screening system to monitor patients during treatment delays realization of personalized treatment. We demonstrate an efficient approach to evaluate drug response using patient-derived circulating tumor cell (CTC) cultures obtained from liquid biopsy. Custom microfabricated tapered microwells were integrated with microfluidics to allow robust formation of CTC clusters without pre-enrichment and subsequent drug screening in situ. Rapid feedback after 2 weeks promotes immediate intervention upon detection of drug resistance or tolerance. The procedure was clinically validated with blood samples (n = 73) from 55 patients with early-stage, newly diagnosed, locally advanced, or refractory metastatic breast cancer. Twenty-four of these samples were used for drug evaluation. Cluster formation potential correlated inversely with increased drug concentration and therapeutic treatment. This new and robust liquid biopsy technique can potentially evaluate patient prognosis with CTC clusters during treatment and provide a noninvasive and inexpensive assessment that can guide drug discovery development or therapeutic choices for personalized treatment.

Research Area(s)

Citation Format(s)

Liquid biopsy and therapeutic response : Circulating tumor cell cultures for evaluation of anticancer treatment. / Khoo, Bee Luan; Grenci, Gianluca; Jing, Tengyang et al.

In: Science Advances, Vol. 2, No. 7, e1600274, 07.2016.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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