Lipo-oligosaccharide profiles of Serpulina pilosicoli strains and their serological cross-reactivities
Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review
Author(s)
Detail(s)
Original language | English |
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Pages (from-to) | 411-415 |
Journal / Publication | Journal of Medical Microbiology |
Volume | 48 |
Issue number | 4 |
Online published | 1 Apr 1999 |
Publication status | Published - Apr 1999 |
Externally published | Yes |
Link(s)
Abstract
The purpose of this study was to determine the presence of lipopolysaccharide-like material in the intestinal spirochaete Serpulina pilosicoli and the extent of antigenic cross-reactivity of this material in different strains of the species. Hot water-phenol, aqueous-phase extracts from five porcine and three human strains of S. pilosicoli, and from seven strains of four other Serpulina spp., were separated by SDS-PAGE and silver-stained profiles were obtained. All S. pilosicoli strains had a predominant band at c. 16 kDa. Some also had a partial ladder-like profile, consistent with the presence of semi-rough lipo-oligosaccharide (LOS); this was more obvious in Western immunoblot analysis. LOS from each S. pilosicoli strain was serologically distinct in immunoblot analysis and there was no cross-reactivity with other Serpulina spp. The serological diversity found amongst the LOS of S. pilosicoli strains may help to explain why individual people and animals can suffer repeated infections with different strains of the organism.
Citation Format(s)
Lipo-oligosaccharide profiles of Serpulina pilosicoli strains and their serological cross-reactivities. / Lee, B. J.; Hampson, D. J.
In: Journal of Medical Microbiology, Vol. 48, No. 4, 04.1999, p. 411-415.
In: Journal of Medical Microbiology, Vol. 48, No. 4, 04.1999, p. 411-415.
Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review