Lipidomic study of olive fruit and oil using TiO2 nanoparticle based matrix solid-phase dispersion and MALDI-TOF/MS
Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review
Author(s)
Detail(s)
Original language | English |
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Pages (from-to) | 2054-2061 |
Journal / Publication | Food Research International |
Volume | 54 |
Issue number | 2 |
Online published | 10 Oct 2013 |
Publication status | Published - Dec 2013 |
Link(s)
Abstract
A matrix solid-phase dispersion (MSPD) procedure, using titanium dioxide (TiO2) nanoparticle (NP) as sorbent, combined with a matrix assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF/MS) method was developed for selective extraction, fast visualization and qualitative analysis of phospholipids from olive fruit and oil samples. The protocol was optimized by testing different sorbents and washing and eluting solvents. The results indicated that clean MS spectrum could be obtained when TiO2 NP was utilized as MSPD sorbent, acidified water (pH5) was used for washing the matrix and enhancing the binding ability, and chloroform and methanol (1/2, v/v) were selected as eluting solvent. In addition, the efficiency of this method was compared with that of the classic Bligh & Dyer method which produced high abundance peaks of glycerides and suppressed the signals of phospholipids. Based on the optimum conditions, olive fruit and oil samples were successfully analyzed in both positive and negative ion modes. The whole method demonstrated to be promising for selective separation of phospholipids from non-phospholipids, and has great potential in fast lipidomic fingerprinting of olive fruits and oils for quality control. © 2013 Elsevier Ltd.
Research Area(s)
- Lipidomics, MALDI-TOF/MS, MSPD, Olive oil, TiO2 nanoparticle
Citation Format(s)
Lipidomic study of olive fruit and oil using TiO2 nanoparticle based matrix solid-phase dispersion and MALDI-TOF/MS. / Shen, Qing; Dong, Wei; Yang, Mei et al.
In: Food Research International, Vol. 54, No. 2, 12.2013, p. 2054-2061.
In: Food Research International, Vol. 54, No. 2, 12.2013, p. 2054-2061.
Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review