Lipidomic profiling of dried seahorses by hydrophilic interaction chromatography coupled to mass spectrometry

Qing Shen; Zhiyuan Dai; Yao-Wen Huang; Hon-Yeung Cheung

doi:10.1016/j.foodchem.2016.02.151

Author(s)

Qing Shen
Zhiyuan Dai
Yao-Wen Huang
Hon-Yeung Cheung

Related Research Unit(s)

Department of Biomedical Sciences

Detail(s)

Original language	English
Pages (from-to)	89-96
Journal / Publication	Food Chemistry
Volume	205
Online published	3 Mar 2016
Publication status	Published - 15 Aug 2016

Link(s)

DOI	DOI https://doi.org/10.1016/j.foodchem.2016.02.151 Final Published version
	Check@CityULib
Link to Scopus	https://www.scopus.com/record/display.uri?eid=2-s2.0-84962290519&origin=recordpage
Permanent Link	https://scholars.cityu.edu.hk/en/publications/publication(c1be8b5b-4fd9-45bb-bf39-162ed2d5c940).html

Abstract

Dried seahorse is a precious raw food material for cooking soups. In this study, a lipidomics strategy using the techniques of solid-phase extraction (SPE) and hydrophilic interaction chromatography-tandem mass spectrometry (HILIC-QTOF/MS) was developed for extraction, visualization, and quantification of phospholipids in dried seahorses. The parameters of SPE were optimized, and 1 mL of sample and chloroform/methanol (1:2, v/v) were found to be the best loading volume and eluting solvent, respectively. Afterwards, each phospholipid class was successfully separated on a HILIC column and analyzed by mass spectrometry. A total of 50 phospholipid molecular species were identified and determined, including 15 phosphatidylcholines (PCs), 14 phosphatidylethanolamines (PEs), 12 phosphatidylinositols (PIs) and 9 phosphatidylserines (PSs). In comparison to previously methods, this strategy was robust and efficient in extraction, characterization, and determination of phospholipids. The dried seahorse was found to contain large amounts of polyunsaturated fatty acyl phospholipids which are beneficial to human health.

Research Area(s)

Hydrophilic interaction chromatography, Lipidomics, Quadruple/time-of-flight mass spectrometry, Seahorse, Solid-phase extraction

Citation Format(s)

[ RIS ] [ BibTeX ]

Lipidomic profiling of dried seahorses by hydrophilic interaction chromatography coupled to mass spectrometry. / Shen, Qing; Dai, Zhiyuan; Huang, Yao-Wen et al.
In: Food Chemistry, Vol. 205, 15.08.2016, p. 89-96.

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

Shen, Q, Dai, Z, Huang, Y-W & Cheung, H-Y 2016, 'Lipidomic profiling of dried seahorses by hydrophilic interaction chromatography coupled to mass spectrometry', Food Chemistry, vol. 205, pp. 89-96. https://doi.org/10.1016/j.foodchem.2016.02.151

Shen, Q., Dai, Z., Huang, Y.-W., & Cheung, H.-Y. (2016). Lipidomic profiling of dried seahorses by hydrophilic interaction chromatography coupled to mass spectrometry. Food Chemistry, 205, 89-96. https://doi.org/10.1016/j.foodchem.2016.02.151

Shen Q, Dai Z, Huang YW, Cheung HY. Lipidomic profiling of dried seahorses by hydrophilic interaction chromatography coupled to mass spectrometry. Food Chemistry. 2016 Aug 15;205:89-96. Epub 2016 Mar 3. doi: 10.1016/j.foodchem.2016.02.151

Shen, Qing ; Dai, Zhiyuan ; Huang, Yao-Wen et al. / Lipidomic profiling of dried seahorses by hydrophilic interaction chromatography coupled to mass spectrometry. In: Food Chemistry. 2016 ; Vol. 205. pp. 89-96.