Linker-free covalent immobilization of heparin, SDF-1α, and CD47 on PTFE surface for antithrombogenicity, endothelialization and anti-inflammation

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal

33 Scopus Citations
View graph of relations

Author(s)

  • Ruiqiang Hang
  • Wei Zhang
  • Penghui Li
  • Long Bai
  • Xue-Feng Yu
  • Huaiyu Wang
  • Liping Tong

Detail(s)

Original languageEnglish
Pages (from-to)201-211
Journal / PublicationBiomaterials
Volume140
Online published20 Jun 2017
Publication statusPublished - Sep 2017

Abstract

Small-diameter vascular grafts made of biomedical polytetrafluoroethylene (PTFE) suffer from the poor long-term patency rate originating from thrombosis and intimal hyperplasia, which can be ascribed to the insufficient endothelialization and chronic inflammation of the materials. Hence, bio-functionalization of PTFE grafts is highly desirable to circumvent these disadvantages. In this study, a versatile “implantation-incubation” approach in which the biomedical PTFE is initially modified by plasma immersion ion implantation (PIII) is described. After the N2 PIII treatment, the surface of biomedical PTFE is roughened with nanostructures and more importantly, the abundant free radicals generated underneath the surface continuously migrate to the surface and react with environmental molecules. Taking advantage of this mechanism, various biomolecules with different functions can be steadily immobilized on the surface of PTFE by simple solution immersion. As examples, three typical biomolecules, heparin, SDF-1α, and CD47, are covalently grafted onto the PTFE. In addition to retaining the bioactivity, the surface-functionalized PTFE exhibits reduced thrombogenicity, facilitates the recruitment of endothelial progenitor cells, and even alleviates the inflammatory immune responses of monocytes-macrophages and is thus promising to the development of small-diameter prosthetic vascular grafts with good long-term patency.

Research Area(s)

  • Covalent immobilization, Multi-functionalization, Plasma immersion ion implantation, PTFE, Surface modification

Citation Format(s)

Linker-free covalent immobilization of heparin, SDF-1α, and CD47 on PTFE surface for antithrombogenicity, endothelialization and anti-inflammation. / Gao, Ang; Hang, Ruiqiang; Li, Wan; Zhang, Wei; Li, Penghui; Wang, Guomin; Bai, Long; Yu, Xue-Feng; Wang, Huaiyu; Tong, Liping; Chu, Paul K.

In: Biomaterials, Vol. 140, 09.2017, p. 201-211.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal