Light Spot-Based Assay for Analysis of Drosophila Larval Phototaxis
Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review
Author(s)
Detail(s)
Original language | English |
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Article number | e60235 |
Journal / Publication | Journal of Visualized Experiments |
Issue number | 151 |
Online published | 27 Sept 2019 |
Publication status | Published - Sept 2019 |
Externally published | Yes |
Link(s)
Abstract
The larvae of Drosophila melanogaster show obvious light-avoiding behavior during the foraging stage. Drosophila larval phototaxis can be used as a model to study animal avoidance behavior. This protocol introduces a light-spot assay to investigate larval phototactic behavior. The experimental set-up includes two main parts: a visual stimulation system that generates the light spot, and an infrared light-based imaging system that records the process of larval light avoidance. This assay allows tracking of the behavior of larva before entering, during encountering, and after leaving the light spot. Details of larval movement including deceleration, pause, head casting, and turning can be captured and analyzed using this method.
Research Area(s)
- Biology, Drosophila, Issue 151, Larva, Light spot assay, Phototaxis, Video recording, Visual system
Citation Format(s)
In: Journal of Visualized Experiments, No. 151, e60235, 09.2019.
Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review