LEPTOSPIRA IN DOGS IN HONG KONG : SEROLOGY AND URINARY SHEDDING

Research output: Chapters, Conference Papers, Creative and Literary WorksRGC 32 - Refereed conference paper (with host publication)peer-review

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Author(s)

  • F Rizzo
  • CL Tsang
  • YR Choi
  • M Staples
  • YM Shuen
  • F Woodhouse
  • Jane Gray
  • VR Barrs

Detail(s)

Original languageEnglish
Title of host publicationJournal of Veterinary Internal Medicine
Pages858
Publication statusPublished - Jan 2024

Publication series

NameJournal of Veterinary Internal Medicine
PublisherWiley Periodicals LLC
Number1
Volume38
ISSN (Print)0891-6640
ISSN (electronic)1939-1676

Conference

Title33rd European Congress of Veterinary Internal Medicine for Companion Animals (ECVIM-CA 2023)
LocationCentre de Convencions Internacional de Barcelona (CCIB)
PlaceSpain
CityBarcelon
Period21 - 23 September 2023

Abstract

Leptospira interrogans causes leptospirosis in dogs and is a zoonotic infection. Vaccination is effective but protection is limited to specific serogroups. This study aimed to determine the prevalence and distribution of Leptospira serogroups and prevalence of urinary shedding in dogs in Hong Kong.
Microagglutination testing (MAT) of 22 serogroups was performed on residual diagnostic sera from 284 owned dogs. Titres above 1:100 were considered positive. Urine from 164 dogs undergoing neutering was tested for pathogenic leptospire DNA by LipL32 quantitative polymerase chain reaction (qPCR). Potential risk factors assessed included signalment, alanine transferase, alkaline phosphatase, bilirubin and creatinine. 
Overall seroprevalence was 9.86% (28/284) and 2.38% of tested dogs (5/210) had leptospiruria (median load 9.30 copies/ng of DNA [range 0.34–232], with a median Ct value of 36.36 [range 30.33–37.00]). In total, 53 positive antibody titres were obtained from 28 seropositive dogs, with 35.71% (10/28) seropositive dogs reactive to >1 serogroup (range 2–6). Fourteen distinct serogroups were detected at titres up to 1:3200, most frequently Pyrogenes (16.98%, 9/53), Copenhageni (13.21%, 7/53), Autumnalis (11.32%, 6/53), Cynopteri (11.32%, 6/53) and Ballum (11.32%, 6/53). Antibodies against vaccination serogroups Canicola (5.66%, 3/53), Pomona (3.77%, 2/53) and Grippotyphosa (3.77%, 2/53) were also detected.
Dogs in Hong Kong are exposed to a wide range of Leptospira serogroups. Current Leptospira vaccines are unlikely protect against the most prevalent serogroups. While prevalence of urinary shedding from dogs is low, gloves should be worn to handle canine urine to prevent zoonotic transmission. 
© 2023 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals LLC on behalf of the American College of Veterinary Internal Medicine.

Citation Format(s)

LEPTOSPIRA IN DOGS IN HONG KONG: SEROLOGY AND URINARY SHEDDING. / Tam, WYJ; Nekouei, O; Rizzo, F et al.
Journal of Veterinary Internal Medicine. 2024. p. 858 (Journal of Veterinary Internal Medicine; Vol. 38, No. 1).

Research output: Chapters, Conference Papers, Creative and Literary WorksRGC 32 - Refereed conference paper (with host publication)peer-review