Left-right asymmetry in cell orientation requires high substrate rigidity

Research output: Chapters, Conference Papers, Creative and Literary Works (RGC: 12, 32, 41, 45)32_Refereed conference paper (with ISBN/ISSN)Not applicablepeer-review

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Author(s)

Detail(s)

Original languageEnglish
Title of host publicationProceedings of the 9th IEEE International Conference on Nano/Molecular Medicine and Engineering
PublisherIEEE
Pages7-11
ISBN (Electronic)978-1-4673-9672-1
StatePublished - Nov 2015

Conference

Title9th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2015
PlaceUnited States
CityHonolulu
Period15 - 18 November 2015

Abstract

Left-right (LR) asymmetry in cell orientation is known as a result of intracellular events such as cytoskeletal organization. However, the dependence of LR asymmetry on mechanical properties of surrounding microenvironment has been unknown. Here we show that the cellular LR behavior was altered when cultured on extracellular matrix with varied substrate rigidity. Using micro-patterned substrate with high rigidity, we found that mouse fibroblasts exhibited a rotational alignment that has significant LR bias, and this LR bias can propagate from the edge to the center of cells. However, when cultured on soft substrate, the LR bias in cell orientation was lost. This finding suggests the importance of combining both intracellular and environment factors when guiding tissue formation, with implication for rebuilding damaged tissue for regenerative medicine.

Research Area(s)

  • left-right asymmetry, micropatterning, rigidity, tissue morphogenesis

Citation Format(s)

Left-right asymmetry in cell orientation requires high substrate rigidity. / Bao, Yuanye; Guo, Zhaobin; Chen, Ting-Hsuan.

Proceedings of the 9th IEEE International Conference on Nano/Molecular Medicine and Engineering. IEEE, 2015. p. 7-11 7492496.

Research output: Chapters, Conference Papers, Creative and Literary Works (RGC: 12, 32, 41, 45)32_Refereed conference paper (with ISBN/ISSN)Not applicablepeer-review