Laser-induced fusion of human embryonic stem cells with optical tweezers

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal

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Author(s)

  • Shuxun Chen
  • Jinping Cheng
  • Chi-Wing Kong
  • Xiaolin Wang
  • Ronald A. Li

Detail(s)

Original languageEnglish
Article number33701
Journal / PublicationApplied Physics Letters
Volume103
Issue number3
Publication statusPublished - 15 Jul 2013

Abstract

We report a study on the laser-induced fusion of human embryonic stem cells (hESCs) at the single-cell level. Cells were manipulated by optical tweezers and fused under irradiation with pulsed UV laser at 355 nm. Successful fusion was indicated by green fluorescence protein transfer. The influence of laser pulse energy on the fusion efficiency was investigated. The fused products were viable as gauged by live cell staining. Successful fusion of hESCs with somatic cells was also demonstrated. The reported fusion outcome may facilitate studies of cell differentiation, maturation, and reprogramming. © 2013 AIP Publishing LLC.

Citation Format(s)

Laser-induced fusion of human embryonic stem cells with optical tweezers. / Chen, Shuxun; Cheng, Jinping; Kong, Chi-Wing; Wang, Xiaolin; Han Cheng, Shuk; Li, Ronald A.; Sun, Dong.

In: Applied Physics Letters, Vol. 103, No. 3, 33701, 15.07.2013.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal