Laser-induced fusion of human embryonic stem cells with optical tweezers

Shuxun Chen; Jinping Cheng; Chi-Wing Kong; Xiaolin Wang; Shuk Han Cheng; Ronald A. Li; Dong Sun

doi:10.1063/1.4813822

Laser-induced fusion of human embryonic stem cells with optical tweezers

Research output: Journal Publications and Reviews (RGC: 21, 22, 62) › 21_Publication in refereed journal › peer-review

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Author(s)

Shuxun Chen
Jinping Cheng
Chi-Wing Kong
Xiaolin Wang
Ronald A. Li

Detail(s)

Original language	English
Article number	33701
Journal / Publication	Applied Physics Letters
Volume	103
Issue number	3
Publication status	Published - 15 Jul 2013

Link(s)

DOI	DOI https://doi.org/10.1063/1.4813822 Final Published version
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Attachment(s)	Documents 5870143.pdf Final Published version, 1.8 MB, PDF Publisher's Copyright Statement This article may be downloaded for personal use only. Any other use requires prior permission of the author and AIP Publishing. This article appeared in Shuxun Chen, Jinping Cheng, Chi-Wing Kong, Xiaolin Wang, Shuk Han Cheng, Ronald A. Li, and Dong Sun , "Laser-induced fusion of human embryonic stem cells with optical tweezers", Appl. Phys. Lett. 103, 033701 (2013) and may be found at https://doi.org/10.1063/1.4813822.
Link to Scopus	https://www.scopus.com/record/display.uri?eid=2-s2.0-84881503135&origin=recordpage
Permanent Link	https://scholars.cityu.edu.hk/en/publications/publication(946d2e6d-049e-4f5e-83cf-2e9904c95596).html

Abstract

We report a study on the laser-induced fusion of human embryonic stem cells (hESCs) at the single-cell level. Cells were manipulated by optical tweezers and fused under irradiation with pulsed UV laser at 355 nm. Successful fusion was indicated by green fluorescence protein transfer. The influence of laser pulse energy on the fusion efficiency was investigated. The fused products were viable as gauged by live cell staining. Successful fusion of hESCs with somatic cells was also demonstrated. The reported fusion outcome may facilitate studies of cell differentiation, maturation, and reprogramming. © 2013 AIP Publishing LLC.