Large field-of-view super-resolution imaging of endo-cellular structures through micro-beads array

Research output: Chapters, Conference Papers, Creative and Literary Works (RGC: 12, 32, 41, 45)32_Refereed conference paper (with ISBN/ISSN)peer-review

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Author(s)

Detail(s)

Original languageEnglish
Title of host publicationIEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED
PublisherIEEE Computer Society
Pages12-16
Volume2016-June
ISBN (Print)9781467396714
Publication statusPublished - 15 Jun 2016

Publication series

Name
Volume2016-June
ISSN (Print)2159-6964
ISSN (Electronic)2159-6972

Conference

Title9th IEEE International Conference on Nano/Molecular Medicine and Engineering (IEEE-NANOMED 2015)
PlaceUnited States
CityHonolulu
Period15 - 18 November 2015

Abstract

This work presents a super-resolution cell imaging method that uses an array of polystyrene (PS) micro-beads lenses embedded in an elastomer thin film on top of a glass substrate. The micro-beads lens array collects reflected near-field light waves from an object's surface and amplifies the waves to allow the formation of enlarged virtual images of the object. We have shown that object images could be magnified up to at least 5 times of the original scale while having a field-of-view of 6 μm (for a single lens), i.e., visible light images of 200 nm features could be captured using a microscope objective lens of 100x (with overall magnification factor of 1000×). Furthermore, using the micro-beads lens array, NIH/3T3 cell internal structures have been successfully imaged under bright-field conditions, showing a much higher resolution image of the internal structures. In summary, we have shown that micro-beads lens array could resolve sub-diffraction-limit features with an overall field-of-view of at least 5000 μm2. Hence, micro-beads lens array shows promising applications in the rapid imaging of large quantity of biomolecules and internal cell structures with wide field-of-view capability.

Research Area(s)

  • biological imaging, micro-beads array, micro-lens array, near-field imaging, super-resolution

Citation Format(s)

Large field-of-view super-resolution imaging of endo-cellular structures through micro-beads array. / Li, Yi; Lai, Hok Sum Sam; Hu, Shuhuan; Lam, Raymond H. W.; Li, Wen Jung.

IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED. Vol. 2016-June IEEE Computer Society, 2016. p. 12-16 7492497.

Research output: Chapters, Conference Papers, Creative and Literary Works (RGC: 12, 32, 41, 45)32_Refereed conference paper (with ISBN/ISSN)peer-review