Labeling enzymes and extracellular polymeric substances in aerobic granules

Chun-Chi Lee; Duu-Jong Lee; Juin-Yih Lai

doi:10.1016/j.jtice.2009.04.002

Author(s)

Chun-Chi Lee
Duu-Jong Lee
Juin-Yih Lai

Detail(s)

Original language	English
Pages (from-to)	505-510
Journal / Publication	Journal of the Taiwan Institute of Chemical Engineers
Volume	40
Issue number	5
Publication status	Published - Sept 2009
Externally published	Yes

Link(s)

DOI	DOI https://doi.org/10.1016/j.jtice.2009.04.002 Final Published version
	Check@CityULib
Link to Scopus	https://www.scopus.com/record/display.uri?eid=2-s2.0-67651175859&origin=recordpage
Permanent Link	https://scholars.cityu.edu.hk/en/publications/publication(e4e7b19c-311e-4d4c-9e4f-ad8f3dc881e5).html

Abstract

Multicolor staining technique provides information on how different constituent components distribute in bioaggregates. This work for the first time adopted immunohistochemical staining and the multicolor fluorescent technique to simultaneously examine the spatial distributions of enzymes, extracellular polymeric substances (EPSs) and cells in aerobic granules. Sample tests with granules cultivated from starch or phenol medium were made to demonstrate the feasibility of the proposed scheme. The cross-talk between channels was revealed in table form. The transient staining test proposed the possible mass transfer limit to the applied antibodies. The proposed staining scheme can appropriately probe the distributions of enzymes, EPS and cells in aerobic granules. © 2009 Taiwan Institute of Chemical Engineers.

Research Area(s)

Aerobic granules, Cross-talk, Enzyme, EPS, Immunohistochemical staining

Bibliographic Note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

Citation Format(s)

[ RIS ] [ BibTeX ]

Labeling enzymes and extracellular polymeric substances in aerobic granules. / Lee, Chun-Chi; Lee, Duu-Jong; Lai, Juin-Yih.
In: Journal of the Taiwan Institute of Chemical Engineers, Vol. 40, No. 5, 09.2009, p. 505-510.

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

Lee, C-C, Lee, D-J & Lai, J-Y 2009, 'Labeling enzymes and extracellular polymeric substances in aerobic granules', Journal of the Taiwan Institute of Chemical Engineers, vol. 40, no. 5, pp. 505-510. https://doi.org/10.1016/j.jtice.2009.04.002

Lee, C.-C., Lee, D.-J., & Lai, J.-Y. (2009). Labeling enzymes and extracellular polymeric substances in aerobic granules. Journal of the Taiwan Institute of Chemical Engineers, 40(5), 505-510. https://doi.org/10.1016/j.jtice.2009.04.002

Lee CC, Lee DJ, Lai JY. Labeling enzymes and extracellular polymeric substances in aerobic granules. Journal of the Taiwan Institute of Chemical Engineers. 2009 Sept;40(5):505-510. doi: 10.1016/j.jtice.2009.04.002

Lee, Chun-Chi ; Lee, Duu-Jong ; Lai, Juin-Yih. / Labeling enzymes and extracellular polymeric substances in aerobic granules. In: Journal of the Taiwan Institute of Chemical Engineers. 2009 ; Vol. 40, No. 5. pp. 505-510.