Label-free enrichment of human blast cells from whole blood for leukemia monitoring

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Original languageEnglish
Article number101584
Journal / PublicationSTAR Protocols
Issue number3
Online published20 Jul 2022
Publication statusPublished - 16 Sept 2022



Liquid biopsy is an alternative to invasive bone marrow biopsy for leukemia detection and management. However, no robust technology is available for enriching leukemic blast cells from the blood. Here, we present a simple and effective protocol for vigorous enrichment of blast cells from whole blood using a one-step microfluidic blast cell biochip (BCB) that exploits distinct cell mechanical properties between diseased and healthy leukocytes. The BCB system achieves higher sensitivity than flow cytometry in detecting blasts. For complete details on the use and execution of this protocol, please refer to Khoo et al. (2019).

Research Area(s)

  • Biotechnology and bioengineering, Cancer, Clinical Protocol, Health Sciences

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