Label-free enrichment of human blast cells from whole blood for leukemia monitoring
Research output: Journal Publications and Reviews (RGC: 21, 22, 62) › 21_Publication in refereed journal › peer-review
Author(s)
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Detail(s)
| Original language | English |
|---|---|
| Article number | 101584 |
| Journal / Publication | STAR Protocols |
| Volume | 3 |
| Issue number | 3 |
| Online published | 20 Jul 2022 |
| Publication status | Published - 16 Sept 2022 |
Link(s)
| DOI | DOI |
|---|---|
| Attachment(s) | Documents
Publisher's Copyright Statement
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| Link to Scopus | https://www.scopus.com/record/display.uri?eid=2-s2.0-85134742503&origin=recordpage |
| Permanent Link | https://scholars.cityu.edu.hk/en/publications/publication(8bddab63-2fc3-440f-b04a-e00c7134a396).html |
Abstract
Liquid biopsy is an alternative to invasive bone marrow biopsy for leukemia detection and management. However, no robust technology is available for enriching leukemic blast cells from the blood. Here, we present a simple and effective protocol for vigorous enrichment of blast cells from whole blood using a one-step microfluidic blast cell biochip (BCB) that exploits distinct cell mechanical properties between diseased and healthy leukocytes. The BCB system achieves higher sensitivity than flow cytometry in detecting blasts. For complete details on the use and execution of this protocol, please refer to Khoo et al. (2019).
Research Area(s)
- Biotechnology and bioengineering, Cancer, Clinical Protocol, Health Sciences
Citation Format(s)
Label-free enrichment of human blast cells from whole blood for leukemia monitoring. / Fu, Yatian; Zou, Shangjie; Khoo, Bee Luan.
In: STAR Protocols, Vol. 3, No. 3, 101584, 16.09.2022.
In: STAR Protocols, Vol. 3, No. 3, 101584, 16.09.2022.
Research output: Journal Publications and Reviews (RGC: 21, 22, 62) › 21_Publication in refereed journal › peer-review
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