Label-free automated three-dimensional imaging of whole organs by microtomy-assisted photoacoustic microscopy

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal

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Author(s)

  • Terence T. W. Wong
  • Ruiying Zhang
  • Chi Zhang
  • Hsun-Chia Hsu
  • Konstantin I. Maslov
  • Junhui Shi
  • Ruimin Chen
  • K. Kirk Shung
  • Qifa Zhou
  • Lihong V. Wang

Detail(s)

Original languageEnglish
Article number1386
Journal / PublicationNature Communications
Volume8
Publication statusPublished - 9 Nov 2017
Externally publishedYes

Link(s)

Abstract

Three-dimensional (3D) optical imaging of whole biological organs with microscopic resolution has remained a challenge. Most versions of such imaging techniques require special preparation of the tissue specimen. Here we demonstrate microtomy-assisted photoacoustic microscopy (mPAM) of mouse brains and other organs, which automatically acquires serial distortion-free and registration-free images with endogenous absorption contrasts. Without tissue staining or clearing, mPAM generates micrometer-resolution 3D images of paraffin- or agarose-embedded whole organs with high fidelity, achieved by label-free simultaneous sensing of DNA/RNA, hemoglobins, and lipids. mPAM provides histology-like imaging of cell nuclei, blood vessels, axons, and other anatomical structures, enabling the application of histopathological interpretation at the organelle level to analyze a whole organ. Its deep tissue imaging capability leads to less sectioning, resulting in negligible sectioning artifact. mPAM offers a new way to better understand complex biological organs.

Research Area(s)

Citation Format(s)

Label-free automated three-dimensional imaging of whole organs by microtomy-assisted photoacoustic microscopy. / Wong, Terence T. W.; Zhang, Ruiying; Zhang, Chi; Hsu, Hsun-Chia; Maslov, Konstantin I.; Wang, Lidai; Shi, Junhui; Chen, Ruimin; Shung, K. Kirk; Zhou, Qifa; Wang, Lihong V.

In: Nature Communications, Vol. 8, 1386, 09.11.2017.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal

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