TY - GEN
T1 - Kinetic and thermodynamic analyses of DNA hybridization reveal the mechanism of gold nanoparticle-assisted single base-pair discrimination in the NanoBioArray chip
AU - Sedighi, Abootaleb
AU - Li, Paul C. H.
N1 - Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].
PY - 2013
Y1 - 2013
N2 - This paper reports the kinetic and thermodynamic studies of DNA hybridization performed in order to comprehend the mechanism of gold nanoparticle (AuNP)-assisted single base-pair discrimination conducted in the NanoBioArray (NBA) chip. In this technique, AuNP-loaded targets DNAs, unlike the target DNAs in the random-coil format, discriminate between perfect matched (PM) probes and the single base-pair mismatched (MM) ones immobilized in the NBA chip surface. Based on our studies, we believe that the binding between target DNA bases and AuNP surfaces shifts the rate-limiting step of both hybridization and dehybridization processes in such a way that favors the mismatch discrimination.
AB - This paper reports the kinetic and thermodynamic studies of DNA hybridization performed in order to comprehend the mechanism of gold nanoparticle (AuNP)-assisted single base-pair discrimination conducted in the NanoBioArray (NBA) chip. In this technique, AuNP-loaded targets DNAs, unlike the target DNAs in the random-coil format, discriminate between perfect matched (PM) probes and the single base-pair mismatched (MM) ones immobilized in the NBA chip surface. Based on our studies, we believe that the binding between target DNA bases and AuNP surfaces shifts the rate-limiting step of both hybridization and dehybridization processes in such a way that favors the mismatch discrimination.
KW - Bioarray chip
KW - DNA dehybridization
KW - DNA hybridization
KW - Gold nanoparticle
KW - Single base-pair discrimination
KW - Transition state theory
KW - Bioarray chip
KW - DNA dehybridization
KW - DNA hybridization
KW - Gold nanoparticle
KW - Single base-pair discrimination
KW - Transition state theory
KW - Bioarray chip
KW - DNA dehybridization
KW - DNA hybridization
KW - Gold nanoparticle
KW - Single base-pair discrimination
KW - Transition state theory
UR - http://www.scopus.com/inward/record.url?scp=84891723411&partnerID=8YFLogxK
UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-84891723411&origin=recordpage
M3 - RGC 32 - Refereed conference paper (with host publication)
SN - 9781632666246
VL - 2
T3 - 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
SP - 865
EP - 867
BT - 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
PB - Chemical and Biological Microsystems Society
T2 - 17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013
Y2 - 27 October 2013 through 31 October 2013
ER -