Japanese encephalitis virus manipulates lysosomes membrane for RNA replication and utilizes autophagy components for intracellular growth

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Qiang Xu
  • Jinchao Xing
  • Jiahao Zhang
  • Huanan Li
  • Lele Liu
  • Chen Hu
  • Ming Liao
  • Wenbao Qi

Related Research Unit(s)

Detail(s)

Original languageEnglish
Article number109025
Journal / PublicationVeterinary Microbiology
Volume255
Online published8 Mar 2021
Publication statusPublished - Apr 2021

Abstract

Japanese encephalitis virus is absolutely dependent on their host cells and has evolved various strategies to manipulate the cellular secretory pathways for viral replication. However, how cellular secretory pathways are hijacked, and the origin of the viral vesicles remains elusive during JEV replication. Here we show how JEV manipulates multiple components of the cellular secretory pathway, including autophagic machinery, to generate a superior environment for genome replication. We utilized double-strand RNA antibodies to label JEV RNA complex seeking the viral replication compartments and found that JEV genome replication takes place in lysosomes (LAMP1), not in autophagosomes (LC3). Subsequently, in situ hybridization results showed that viral RNAs (vRNAs) of JEV strongly colocalized with LAMP1. What surprised us was that JEV vRNAs markedly colocalized with LC3, indicating that autophagy plays an active role in JEV replication. Interestingly, we found that JEV utilized autophagic components for intracellular growth in an autophagy-dependent manner and the fusion of autophagosome-lysosome plays a positive role in JEV post-RNA replication processes. Collectively, our findings demonstrate that JEV can manipulate cellular secretory pathway to form genome replication organelles and exploit autophagy components for intracellular growth, providing new insights into the life cycle of JEV and uncovering an attractive target for antiviral drugs.

Research Area(s)

  • Autophagy, Cellular secretory pathway, Japanese encephalitis virus, Lysosomes, RNA replication

Citation Format(s)

Japanese encephalitis virus manipulates lysosomes membrane for RNA replication and utilizes autophagy components for intracellular growth. / Xu, Qiang; Huang, Lihong; Xing, Jinchao; Zhang, Jiahao; Li, Huanan; Liu, Lele; Hu, Chen; Liao, Ming; Yue, Jianbo; Qi, Wenbao.

In: Veterinary Microbiology, Vol. 255, 109025, 04.2021.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review