TY - JOUR
T1 - Trichosporon asahii
T2 - A Potential Growth Promoter for C. gallinacea? Implications for Chlamydial Infections and Cell Culture
AU - Ornelas-Eusebio, Erika
AU - Vorimore, Fabien
AU - Aaziz, Rachid
AU - Mandola, Maria-Lucia
AU - Rizzo, Francesca
AU - Marchino, Monica
AU - Nogarol, Chiara
AU - Risco-Castillo, Veronica
AU - Zanella, Gina
AU - Schnee, Christiane
AU - Sachse, Konrad
AU - Laroucau, Karine
PY - 2025/2
Y1 - 2025/2
N2 - The cultivation of Chlamydia gallinacea, a recently identified species, is challenging due to the lack of an optimized protocol. In this study, several infection protocols were tested, including different cell lines, incubation temperatures, centrifugation methods and culture media. However, none were successful in field samples. The only exception was a chance co-culture with Trichosporon asahii, a microorganism commonly found in the chicken gut. This suggests that current in vitro methods may not be optimized for this species and that host-associated microorganisms may influence the in vivo growth of C. gallinacea, which is typically found in the chicken gut. These findings raise new questions and highlight the need for further investigation of microbial interactions within the host, particularly to understand their role in the proliferation of chlamydial species. © 2025 by the authors.
AB - The cultivation of Chlamydia gallinacea, a recently identified species, is challenging due to the lack of an optimized protocol. In this study, several infection protocols were tested, including different cell lines, incubation temperatures, centrifugation methods and culture media. However, none were successful in field samples. The only exception was a chance co-culture with Trichosporon asahii, a microorganism commonly found in the chicken gut. This suggests that current in vitro methods may not be optimized for this species and that host-associated microorganisms may influence the in vivo growth of C. gallinacea, which is typically found in the chicken gut. These findings raise new questions and highlight the need for further investigation of microbial interactions within the host, particularly to understand their role in the proliferation of chlamydial species. © 2025 by the authors.
KW - Chlamydia gallinacea
KW - cultivation
KW - optimization
KW - Trichosporon
UR - http://www.scopus.com/inward/record.url?scp=85219568316&partnerID=8YFLogxK
UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-85219568316&origin=recordpage
U2 - 10.3390/microorganisms13020288
DO - 10.3390/microorganisms13020288
M3 - RGC 21 - Publication in refereed journal
SN - 2076-2607
VL - 13
JO - Microorganisms
JF - Microorganisms
IS - 2
M1 - 288
ER -