Isolation and retrieval of circulating tumor cells using centrifugal forces

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Han Wei Hou
  • Majid Ebrahimi Warkiani
  • Zi Rui Li
  • Ross A. Soo
  • Daniel Shao-Weng Tan
  • Wan-Teck Lim
  • Jongyoon Han
  • Ali Asgar S. Bhagat
  • Chwee Teck Lim

Detail(s)

Original languageEnglish
Article number1259
Journal / PublicationScientific Reports
Volume3
Online published12 Feb 2013
Publication statusPublished - 2013
Externally publishedYes

Link(s)

Abstract

Presence and frequency of rare circulating tumor cells (CTCs) in bloodstreams of cancer patients are pivotal to early cancer detection and treatment monitoring. Here, we use a spiral microchannel with inherent centrifugal forces for continuous, size-based separation of CTCs from blood (Dean Flow Fractionation (DFF)) which facilitates easy coupling with conventional downstream biological assays. Device performance was optimized using cancer cell lines (> 85% recovery), followed by clinical validation with positive CTCs enumeration in all samples from patients with metastatic lung cancer (n = 20; 5-88 CTCs per mL). The presence of CD133+ cells, a phenotypic marker characteristic of stem-like behavior in lung cancer cells was also identified in the isolated subpopulation of CTCs. The spiral biochip identifies and addresses key challenges of the next generation CTCs isolation assay including antibody independent isolation, high sensitivity and throughput (3 mL/hr); and single-step retrieval of viable CTCs.

Research Area(s)

Citation Format(s)

Isolation and retrieval of circulating tumor cells using centrifugal forces. / Hou, Han Wei; Warkiani, Majid Ebrahimi; Khoo, Bee Luan et al.

In: Scientific Reports, Vol. 3, 1259, 2013.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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