Investigation of a PDMS based micromixer for heterogeneous immunoassays of insulin

Zhikun Zhan; Yanli Qu; Shuai Wang; Zaili Dong; Balaji Srinivasan; Steve Tung; Wen J. Li

doi:10.1109/NEMS.2010.5592478

Investigation of a PDMS based micromixer for heterogeneous immunoassays of insulin

Zhikun Zhan, Yanli Qu, Shuai Wang, Zaili Dong, Balaji Srinivasan, Steve Tung, Wen J. Li

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

1 Citation (Scopus)

Abstract

An experimental study was carried out to evaluate the performance of a polydimethylsiloxane (PDMS) based micromixer for heterogeneous immunoassays of insulin. The detection method is based on coupling the highly specific double-antibody sandwich immunoassay with the sensitive chemiluminescence of the Luminol-Hydrogen Peroxide reaction. Both the immune and chemiluminescence reactions are conducted in the micromixer, and the resultant light emission is detected by a highly sensitive photometer. The behavior of the luminol based chemiluminescence is parabolic with respect to time, and the maximum light intensity is used here to represent the output of the reaction. The current results indicate that at an actuation pressure of 15psi, an actuation frequency of 30Hz leads to the best reaction result. At these operating conditions, the detection limit of insulin is about 10^-14mol/L while the total reaction time of the insulin immunoassay in the micromixer is less than 5 minutes. ©2010 IEEE.

Original language	English
Title of host publication	2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010
Pages	616-619
DOIs	https://doi.org/10.1109/NEMS.2010.5592478
Publication status	Published - 2010
Externally published	Yes
Event	5th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (IEEE-NEMS 2010) - Xiamen, China Duration: 20 Jan 2010 → 23 Jan 2010

Conference

Conference	5th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (IEEE-NEMS 2010)
Abbreviated title	IEEE-NEMS 2010
Place	China
City	Xiamen
Period	20/01/10 → 23/01/10

Research Keywords

Immunoassay
Insulin
Micromixer
PDMS

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10.1109/NEMS.2010.5592478

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title = "Investigation of a PDMS based micromixer for heterogeneous immunoassays of insulin",

abstract = "An experimental study was carried out to evaluate the performance of a polydimethylsiloxane (PDMS) based micromixer for heterogeneous immunoassays of insulin. The detection method is based on coupling the highly specific double-antibody sandwich immunoassay with the sensitive chemiluminescence of the Luminol-Hydrogen Peroxide reaction. Both the immune and chemiluminescence reactions are conducted in the micromixer, and the resultant light emission is detected by a highly sensitive photometer. The behavior of the luminol based chemiluminescence is parabolic with respect to time, and the maximum light intensity is used here to represent the output of the reaction. The current results indicate that at an actuation pressure of 15psi, an actuation frequency of 30Hz leads to the best reaction result. At these operating conditions, the detection limit of insulin is about 10-14mol/L while the total reaction time of the insulin immunoassay in the micromixer is less than 5 minutes. {\textcopyright}2010 IEEE.",

keywords = "Immunoassay, Insulin, Micromixer, PDMS",

author = "Zhikun Zhan and Yanli Qu and Shuai Wang and Zaili Dong and Balaji Srinivasan and Steve Tung and Li, {Wen J.}",

year = "2010",

doi = "10.1109/NEMS.2010.5592478",

language = "English",

isbn = "9781424465439",

pages = "616--619",

booktitle = "2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010",

note = "5th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (IEEE-NEMS 2010), IEEE-NEMS 2010 ; Conference date: 20-01-2010 Through 23-01-2010",

}

Zhan, Z, Qu, Y, Wang, S, Dong, Z, Srinivasan, B, Tung, S & Li, WJ 2010, Investigation of a PDMS based micromixer for heterogeneous immunoassays of insulin. in 2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010., 5592478, pp. 616-619, 5th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (IEEE-NEMS 2010), Xiamen, China, 20/01/10. https://doi.org/10.1109/NEMS.2010.5592478

Investigation of a PDMS based micromixer for heterogeneous immunoassays of insulin. / Zhan, Zhikun; Qu, Yanli; Wang, Shuai et al.
2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010. 2010. p. 616-619 5592478.

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

TY - GEN

T1 - Investigation of a PDMS based micromixer for heterogeneous immunoassays of insulin

AU - Zhan, Zhikun

AU - Qu, Yanli

AU - Wang, Shuai

AU - Dong, Zaili

AU - Srinivasan, Balaji

AU - Tung, Steve

AU - Li, Wen J.

PY - 2010

Y1 - 2010

N2 - An experimental study was carried out to evaluate the performance of a polydimethylsiloxane (PDMS) based micromixer for heterogeneous immunoassays of insulin. The detection method is based on coupling the highly specific double-antibody sandwich immunoassay with the sensitive chemiluminescence of the Luminol-Hydrogen Peroxide reaction. Both the immune and chemiluminescence reactions are conducted in the micromixer, and the resultant light emission is detected by a highly sensitive photometer. The behavior of the luminol based chemiluminescence is parabolic with respect to time, and the maximum light intensity is used here to represent the output of the reaction. The current results indicate that at an actuation pressure of 15psi, an actuation frequency of 30Hz leads to the best reaction result. At these operating conditions, the detection limit of insulin is about 10-14mol/L while the total reaction time of the insulin immunoassay in the micromixer is less than 5 minutes. ©2010 IEEE.

AB - An experimental study was carried out to evaluate the performance of a polydimethylsiloxane (PDMS) based micromixer for heterogeneous immunoassays of insulin. The detection method is based on coupling the highly specific double-antibody sandwich immunoassay with the sensitive chemiluminescence of the Luminol-Hydrogen Peroxide reaction. Both the immune and chemiluminescence reactions are conducted in the micromixer, and the resultant light emission is detected by a highly sensitive photometer. The behavior of the luminol based chemiluminescence is parabolic with respect to time, and the maximum light intensity is used here to represent the output of the reaction. The current results indicate that at an actuation pressure of 15psi, an actuation frequency of 30Hz leads to the best reaction result. At these operating conditions, the detection limit of insulin is about 10-14mol/L while the total reaction time of the insulin immunoassay in the micromixer is less than 5 minutes. ©2010 IEEE.

KW - Immunoassay

KW - Insulin

KW - Micromixer

KW - PDMS

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DO - 10.1109/NEMS.2010.5592478

M3 - RGC 32 - Refereed conference paper (with host publication)

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EP - 619

BT - 2010 IEEE 5th International Conference on Nano/Micro Engineered and Molecular Systems, NEMS 2010

T2 - 5th IEEE International Conference on Nano/Micro Engineered and Molecular Systems (IEEE-NEMS 2010)

Y2 - 20 January 2010 through 23 January 2010

ER -

Investigation of a PDMS based micromixer for heterogeneous immunoassays of insulin

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