Intradermal delivery of mRNA using cryomicroneedles

Jinming Yu; Chaiyaporn Kuwentrai; Hua-Rui Gong; Renhao Li; Bao-zhong Zhang; Xuansheng Lin; Xiaolei Wang; Jian-Dong Huang; Chenjie Xu

doi:10.1016/j.actbio.2022.06.015

Intradermal delivery of mRNA using cryomicroneedles

Jinming Yu, Chaiyaporn Kuwentrai, Hua-Rui Gong, Renhao Li, Bao-zhong Zhang, Xuansheng Lin, Xiaolei Wang, Jian-Dong Huang^*, Chenjie Xu^*

^*Corresponding author for this work

Department of Biomedical Engineering

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

38 Citations (Scopus)

Abstract

Microneedles can realize the intradermal and transdermal delivery of drugs. However, most conventional microneedles made of metal, polymer and ceramics are unsuitable for the delivery of mRNA drugs that are fragile and temperature sensitive. This study explores the usage of cryomicroneedles (CryoMNs) for the intradermal delivery of mRNA molecules. Taking luciferase mRNA as an example, we first optimize the formulation of CryoMNs to maximize mRNA stability. Later, in the mice model, we compare the delivery efficiency with the conventional subcutaneous injection for both the luciferase mRNA and COVID-19 Comirnaty mRNA vaccines, where CryoMN delivered mRNA vaccines successfully induce specific B cell antibody, neutralizing activity and T cell responses.

Original language	English
Pages (from-to)	133-141
Journal	Acta Biomaterialia
Volume	148
Online published	11 Jun 2022
DOIs	https://doi.org/10.1016/j.actbio.2022.06.015
Publication status	Published - Aug 2022

Research Keywords

Cryomicroneedle
Intradermal delivery
mRNA
Comirnaty mRNA vaccines

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.actbio.2022.06.015

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title = "Intradermal delivery of mRNA using cryomicroneedles",

abstract = "Microneedles can realize the intradermal and transdermal delivery of drugs. However, most conventional microneedles made of metal, polymer and ceramics are unsuitable for the delivery of mRNA drugs that are fragile and temperature sensitive. This study explores the usage of cryomicroneedles (CryoMNs) for the intradermal delivery of mRNA molecules. Taking luciferase mRNA as an example, we first optimize the formulation of CryoMNs to maximize mRNA stability. Later, in the mice model, we compare the delivery efficiency with the conventional subcutaneous injection for both the luciferase mRNA and COVID-19 Comirnaty mRNA vaccines, where CryoMN delivered mRNA vaccines successfully induce specific B cell antibody, neutralizing activity and T cell responses.",

keywords = "Cryomicroneedle, Intradermal delivery, mRNA, Comirnaty mRNA vaccines",

author = "Jinming Yu and Chaiyaporn Kuwentrai and Hua-Rui Gong and Renhao Li and Bao-zhong Zhang and Xuansheng Lin and Xiaolei Wang and Jian-Dong Huang and Chenjie Xu",

year = "2022",

month = aug,

doi = "10.1016/j.actbio.2022.06.015",

language = "English",

volume = "148",

pages = "133--141",

journal = "Acta Biomaterialia",

issn = "1742-7061",

publisher = "Elsevier Ltd.",

}

TY - JOUR

T1 - Intradermal delivery of mRNA using cryomicroneedles

AU - Yu, Jinming

AU - Kuwentrai, Chaiyaporn

AU - Gong, Hua-Rui

AU - Li, Renhao

AU - Zhang, Bao-zhong

AU - Lin, Xuansheng

AU - Wang, Xiaolei

AU - Huang, Jian-Dong

AU - Xu, Chenjie

PY - 2022/8

Y1 - 2022/8

N2 - Microneedles can realize the intradermal and transdermal delivery of drugs. However, most conventional microneedles made of metal, polymer and ceramics are unsuitable for the delivery of mRNA drugs that are fragile and temperature sensitive. This study explores the usage of cryomicroneedles (CryoMNs) for the intradermal delivery of mRNA molecules. Taking luciferase mRNA as an example, we first optimize the formulation of CryoMNs to maximize mRNA stability. Later, in the mice model, we compare the delivery efficiency with the conventional subcutaneous injection for both the luciferase mRNA and COVID-19 Comirnaty mRNA vaccines, where CryoMN delivered mRNA vaccines successfully induce specific B cell antibody, neutralizing activity and T cell responses.

AB - Microneedles can realize the intradermal and transdermal delivery of drugs. However, most conventional microneedles made of metal, polymer and ceramics are unsuitable for the delivery of mRNA drugs that are fragile and temperature sensitive. This study explores the usage of cryomicroneedles (CryoMNs) for the intradermal delivery of mRNA molecules. Taking luciferase mRNA as an example, we first optimize the formulation of CryoMNs to maximize mRNA stability. Later, in the mice model, we compare the delivery efficiency with the conventional subcutaneous injection for both the luciferase mRNA and COVID-19 Comirnaty mRNA vaccines, where CryoMN delivered mRNA vaccines successfully induce specific B cell antibody, neutralizing activity and T cell responses.

KW - Cryomicroneedle

KW - Intradermal delivery

KW - mRNA

KW - Comirnaty mRNA vaccines

UR - http://www.scopus.com/inward/record.url?scp=85132846453&partnerID=8YFLogxK

UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-85132846453&origin=recordpage

U2 - 10.1016/j.actbio.2022.06.015

DO - 10.1016/j.actbio.2022.06.015

M3 - RGC 21 - Publication in refereed journal

SN - 1742-7061

VL - 148

SP - 133

EP - 141

JO - Acta Biomaterialia

JF - Acta Biomaterialia

ER -

Intradermal delivery of mRNA using cryomicroneedles

Abstract

Research Keywords

UN SDGs

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GRF: Development of Framework Nucleic Acid based platform technology for intradermal and transdermal delivery of oligonucleotides therapeutics

GRF: Development of Highly Swellable Microneedle Skin Patches for Skin Interstitial Fluid Sampling and In Situ Transdermal Biosensing

NSFC: Nanometallic Conductive Composite-hydrogel Core-shell Microneedle Skin Patch for Real-time Monitoring Physiological Signals

Cite this

Intradermal delivery of mRNA using cryomicroneedles

Abstract

Research Keywords

UN SDGs

Access Output

Other Access Options

Permanent Link

Other Files and Links

Fingerprint

Projects

GRF: Development of Framework Nucleic Acid based platform technology for intradermal and transdermal delivery of oligonucleotides therapeutics

GRF: Development of Highly Swellable Microneedle Skin Patches for Skin Interstitial Fluid Sampling and In Situ Transdermal Biosensing

NSFC: Nanometallic Conductive Composite-hydrogel Core-shell Microneedle Skin Patch for Real-time Monitoring Physiological Signals

Cite this