Intracellular chromosome breaks on silicon surface

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal

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Author(s)

  • Jiang Jiang
  • Kaifu Huo
  • Shaopeng Chen
  • Yunchang Xin
  • Yongjian Xu
  • Zhengwei Wu
  • Zengliang Yu

Detail(s)

Original languageEnglish
Pages (from-to)2661-2665
Journal / PublicationBiomaterials
Volume30
Issue number14
Publication statusPublished - May 2009

Abstract

The genotoxicity of silicon (Si) is investigated by soaking crystalline Si in a complete culture medium for 60 days and conducting micronuclei tests (MNTs) utilizing hamster ovary (CHO) cells and its Ku80 deficient CHO mutant (xrs5) cells (DNA double-strand breaks repair deficiency). The intracellular concentrations of reactive oxygen/nitrogen species (ROS/RNS) on Si are determined to elucidate the relationship between ROS/RNS and Si-induced genotoxicity by using CHO cells. The cells are treated with ROS scavenger (dimethyl sulfoxide) and MNT are performed. The results indicate that the intracellular concentration of ROS and nitrogen oxide (NO) on Si is higher than those on the control group by about 38% and 12%. ROS/RNS include superoxide (O2{radical dot}-) anion, NO, and peroxynitrite (ONOO-) which can injure chromosomes and induce high cellular DNA double-strand breaks (DSBs). © 2009 Elsevier Ltd. All rights reserved.

Research Area(s)

  • Biocompatibility, DNA double-strand breaks, Micronuclei, Reactive oxygen/nitrogen species, Silicon

Citation Format(s)

Intracellular chromosome breaks on silicon surface. / Jiang, Jiang; Huo, Kaifu; Chen, Shaopeng; Xin, Yunchang; Xu, Yongjian; Wu, Zhengwei; Yu, Zengliang; Chu, Paul K.

In: Biomaterials, Vol. 30, No. 14, 05.2009, p. 2661-2665.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journal