Interactions of paralytic shellfish toxins with xenobiotic-metabolizing and antioxidant enzymes in rodents

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Hai-Zheng Hong
  • Paul K.S. Lam
  • Dennis P.H. Hsieh

Detail(s)

Original languageEnglish
Pages (from-to)425-431
Journal / PublicationToxicon
Volume42
Issue number4
Publication statusPublished - Sep 2003

Abstract

Paralytic shellfish toxins (PSTs) are neurotoxins known to block voltage-gated sodium channels in intoxicated animals and humans. Their metabolism in mammalian systems and their effects on other receptors are not as well understood. In this study, we investigated the in vitro metabolism of two classes of PSTs, gonyautoxin 2/3 (GTX2/3) and C1/2 toxins (C1/2), using rat and mouse liver enzyme preparations. We also analyzed the effects of these toxins on several antioxidant and xenobiotic-metabolizing enzymes in mice. These toxins were selected for their prevalence in the coastal waters of Southern China. When the toxins were incubated with liver preparations containing Phase I and Phase II xenobiotic metabolizing enzymes and appropriate co-factors, no transformation of the toxins was detectable. When mice were given sub-lethal doses of GTX2/3, a loss of activity was observed in hepatic ethoxyresorufin-O-deethylase, penthoxyresorufin-O-deethylase, glutathione peroxidase and superoxide dismutase, but not in glutathione S-transferase, catalase and glutathione reductase. Exposure to the same mouse units of C1/2 caused only a slight reduction in the activity of penthoxyresorufin-O-deethylase and glutathione peroxidase. Our results indicated that these toxins may not be metabolized readily in mammals and that they may cause adverse effects other than sodium channel blocking. © 2003 Elsevier Ltd. All rights reserved.

Research Area(s)

  • Antioxidant enzymes, C1/2 toxins, Gonyautoxin 2/3, Metabolic transformation, Paralytic shellfish toxins, Rodents, Xenobiotic metabolizing

Citation Format(s)

Interactions of paralytic shellfish toxins with xenobiotic-metabolizing and antioxidant enzymes in rodents. / Hong, Hai-Zheng; Lam, Paul K.S.; Hsieh, Dennis P.H.

In: Toxicon, Vol. 42, No. 4, 09.2003, p. 425-431.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review