Interaction between the fluorinated amphiphilic copolymer poly(2,2,3,4,4,4-hexafluorobutyl methacrylate)-graft-poly(SPEG) and DNA

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Ling Li
  • Shengdong Xiong
  • Yingxi Wang
  • Gongwu Song
  • Shuilin Wu
  • Zushun Xu

Detail(s)

Original languageEnglish
Pages (from-to)291-298
Journal / PublicationJournal of Applied Polymer Science
Volume118
Issue number1
Publication statusPublished - 5 Oct 2010

Abstract

A synthesized copolymer, synthesized from HFMA (hexaflurobutyl methacrylate) and SPEG (PHFMA-g-PSPEG), was synthesized. PHFMA- g-PSPEG intercalated to the DNA base pair via a strong hydrophobic force, and this was conformed by ultraviolet spectroscopy, transmittance measurements, micropolarity measurements, resonance light scattering (RLS) spectroscopy, and particle size measurements. The copolymer was used as a new probe to detect DNA according to the RLS technique. The hydrophobic interaction between PHFMA-g-PSPEG and DNA significantly enhanced the RLS signal, and the enhanced RLS intensity at 422 nm was proportional to the nucleic acid concentration within the range of 0.09-0.90 mg/L with a detection limit (3σ) of 4.0 μg/L. © 2010 Wiley Periodicals, Inc.

Research Area(s)

  • Biological applications of polymers, Drug delivery systems, Fluoropolymers

Citation Format(s)

Interaction between the fluorinated amphiphilic copolymer poly(2,2,3,4,4,4-hexafluorobutyl methacrylate)-graft-poly(SPEG) and DNA. / Li, Ling; Xiong, Shengdong; Wang, Yingxi; Song, Gongwu; Wu, Shuilin; Chu, Paul K.; Xu, Zushun.

In: Journal of Applied Polymer Science, Vol. 118, No. 1, 05.10.2010, p. 291-298.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review