Integrative platform for ultrahigh throughput quantitative mechanoresponse of adhered single cells

Ming Wang; Hwa Liang Leo; Chwee Teck Lim; Chia-Hung Chen

Integrative platform for ultrahigh throughput quantitative mechanoresponse of adhered single cells

Ming Wang
, Hwa Liang Leo
, Chwee Teck Lim
, Chia-Hung Chen^*

^*Corresponding author for this work

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

Abstract

Extracellular matrix (ECM) is essential for most cell types. Mechanical properties of ECM are pivotal in cytoskeleton development and in influencing a variety of cell phenotypes. For instance, individual responses of cancer cells to mechanical cues can significantly influence cancer progression; thus it is essential to examine single cellular phenotypic mechanoresponse to provide insights into disease progression. In this study, a novel screening technique has been developed in which individual single cells adhered onto a hydrogel particle and screened in a continuous flow manner.

Original language	English
Title of host publication	22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2018)
Publisher	Chemical and Biological Microsystems Society
Pages	349-351
Volume	1
ISBN (Electronic)	9780578405308
ISBN (Print)	9781510897571
Publication status	Published - Nov 2018
Externally published	Yes
Event	22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2018) - Kaohsiung Exhibition Center, Kaohsiung, Taiwan, China Duration: 11 Nov 2018 → 15 Nov 2018 https://cbmsociety.org/microtas/microtas2018/ https://cbmsociety.org/wp-content/uploads/2018/11/uTAS-2018-FInal-Program-WEB.pdf https://archive.cbmsociety.org/utas/uTAS2018.zip

Publication series

Name	International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS
Publisher	Chemical and Biological Microsystems Society
ISSN (Electronic)	1556-5904

Conference

Conference	22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2018)
Abbreviated title	μTAS2018
Place	Taiwan, China
City	Kaohsiung
Period	11/11/18 → 15/11/18
Internet address	https://cbmsociety.org/microtas/microtas2018/ https://cbmsociety.org/wp-content/uploads/2018/11/uTAS-2018-FInal-Program-WEB.pdf https://archive.cbmsociety.org/utas/uTAS2018.zip

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Research Keywords

Adhered cell
Cell-matrix interaction
High throughput screening
Secretomics
Single cell assay

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Wang, M., Leo, H. L., Lim, C. T., & Chen, C.-H. (2018). Integrative platform for ultrahigh throughput quantitative mechanoresponse of adhered single cells. In 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2018) (Vol. 1, pp. 349-351). (International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS). Chemical and Biological Microsystems Society.

Wang, Ming ; Leo, Hwa Liang ; Lim, Chwee Teck et al. / Integrative platform for ultrahigh throughput quantitative mechanoresponse of adhered single cells. 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2018). Vol. 1 Chemical and Biological Microsystems Society, 2018. pp. 349-351 (International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS).

@inproceedings{c91d4da0560d44f5bc4c67a235f871a9,

title = "Integrative platform for ultrahigh throughput quantitative mechanoresponse of adhered single cells",

abstract = "Extracellular matrix (ECM) is essential for most cell types. Mechanical properties of ECM are pivotal in cytoskeleton development and in influencing a variety of cell phenotypes. For instance, individual responses of cancer cells to mechanical cues can significantly influence cancer progression; thus it is essential to examine single cellular phenotypic mechanoresponse to provide insights into disease progression. In this study, a novel screening technique has been developed in which individual single cells adhered onto a hydrogel particle and screened in a continuous flow manner.",

keywords = "Adhered cell, Cell-matrix interaction, High throughput screening, Secretomics, Single cell assay",

author = "Ming Wang and Leo, \{Hwa Liang\} and Lim, \{Chwee Teck\} and Chia-Hung Chen",

year = "2018",

month = nov,

language = "English",

isbn = "9781510897571",

volume = "1",

series = "International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS",

publisher = "Chemical and Biological Microsystems Society",

pages = "349--351",

booktitle = "22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2018)",

note = "22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2018), μTAS2018 ; Conference date: 11-11-2018 Through 15-11-2018",

url = "https://cbmsociety.org/microtas/microtas2018/, https://cbmsociety.org/wp-content/uploads/2018/11/uTAS-2018-FInal-Program-WEB.pdf, https://archive.cbmsociety.org/utas/uTAS2018.zip",

}

Wang, M, Leo, HL, Lim, CT & Chen, C-H 2018, Integrative platform for ultrahigh throughput quantitative mechanoresponse of adhered single cells. in 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2018). vol. 1, International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS, Chemical and Biological Microsystems Society, pp. 349-351, 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2018), Kaohsiung, Taiwan, China, 11/11/18.

Integrative platform for ultrahigh throughput quantitative mechanoresponse of adhered single cells. / Wang, Ming; Leo, Hwa Liang; Lim, Chwee Teck et al.
22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2018). Vol. 1 Chemical and Biological Microsystems Society, 2018. p. 349-351 (International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS).

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

TY - GEN

T1 - Integrative platform for ultrahigh throughput quantitative mechanoresponse of adhered single cells

AU - Wang, Ming

AU - Leo, Hwa Liang

AU - Lim, Chwee Teck

AU - Chen, Chia-Hung

PY - 2018/11

Y1 - 2018/11

N2 - Extracellular matrix (ECM) is essential for most cell types. Mechanical properties of ECM are pivotal in cytoskeleton development and in influencing a variety of cell phenotypes. For instance, individual responses of cancer cells to mechanical cues can significantly influence cancer progression; thus it is essential to examine single cellular phenotypic mechanoresponse to provide insights into disease progression. In this study, a novel screening technique has been developed in which individual single cells adhered onto a hydrogel particle and screened in a continuous flow manner.

AB - Extracellular matrix (ECM) is essential for most cell types. Mechanical properties of ECM are pivotal in cytoskeleton development and in influencing a variety of cell phenotypes. For instance, individual responses of cancer cells to mechanical cues can significantly influence cancer progression; thus it is essential to examine single cellular phenotypic mechanoresponse to provide insights into disease progression. In this study, a novel screening technique has been developed in which individual single cells adhered onto a hydrogel particle and screened in a continuous flow manner.

KW - Adhered cell

KW - Cell-matrix interaction

KW - High throughput screening

KW - Secretomics

KW - Single cell assay

UR - https://www.scopus.com/pages/publications/85079845737

UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-85079845737&origin=recordpage

M3 - RGC 32 - Refereed conference paper (with host publication)

SN - 9781510897571

VL - 1

T3 - International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS

SP - 349

EP - 351

BT - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2018)

PB - Chemical and Biological Microsystems Society

T2 - 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2018)

Y2 - 11 November 2018 through 15 November 2018

ER -

Wang M, Leo HL, Lim CT, Chen CH. Integrative platform for ultrahigh throughput quantitative mechanoresponse of adhered single cells. In 22nd International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2018). Vol. 1. Chemical and Biological Microsystems Society. 2018. p. 349-351. (International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS).

Integrative platform for ultrahigh throughput quantitative mechanoresponse of adhered single cells

Abstract

Publication series

Conference

UN SDGs

Research Keywords

Other Access Options

Permanent Link

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Cite this