TY - GEN
T1 - Integrated microfluidic system for rapid selection of CRP aptamers utilizing systematic evolution of ligands by exponentail enrichment
AU - Huang, Chao-June
AU - Lin, Hsin-I
AU - Shiesh, Shu-Chu
AU - Lee, Gwo-Bin
N1 - Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].
PY - 2009
Y1 - 2009
N2 - Systematic evolution of ligands by exponential enrichment (SELEX) has been a crucial procedure which allows extraction of oligomers with desired binding affinity for a target molecule from an initially random pool of oligonucleotides. It can be used to screen DNA- or RNA-based aptamers which are specific to proteins and biomolecules, making it a promising platform for the study of biomarkers. This study reports a new magnetic-bead-based microfluidic system for performing SELEX in an automatic mode. The developed microsystem is promising for fast selection of aptamers, which is an excellent tool for selection of nucleotides with high affinity for a particular target from a random pool under specific conditions. © 2009 CBMS.
AB - Systematic evolution of ligands by exponential enrichment (SELEX) has been a crucial procedure which allows extraction of oligomers with desired binding affinity for a target molecule from an initially random pool of oligonucleotides. It can be used to screen DNA- or RNA-based aptamers which are specific to proteins and biomolecules, making it a promising platform for the study of biomarkers. This study reports a new magnetic-bead-based microfluidic system for performing SELEX in an automatic mode. The developed microsystem is promising for fast selection of aptamers, which is an excellent tool for selection of nucleotides with high affinity for a particular target from a random pool under specific conditions. © 2009 CBMS.
KW - Aptamers
KW - Microfluidics
KW - PCR
KW - SELEX
UR - https://www.scopus.com/pages/publications/77952784331#tab=impact
UR - https://www.scopus.com/pages/publications/77952784331
M3 - RGC 32 - Refereed conference paper (with host publication)
SN - 9780979806421
T3 - Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
SP - 150
EP - 152
BT - Proceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PB - Chemical and Biological Microsystems Society
T2 - 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
Y2 - 1 November 2009 through 5 November 2009
ER -