Integrated microfluidic system for HIV detection

Jung-Hao Wang; Chih-Hung Wang; Wei-Shuo Ling; Lie Jheng; Shainn-Wei Wang; Gwo-Bin Lee

doi:10.1109/MEMSYS.2012.6170346

Integrated microfluidic system for HIV detection

Jung-Hao Wang
, Chih-Hung Wang
, Wei-Shuo Ling
, Lie Jheng
, Shainn-Wei Wang
, Gwo-Bin Lee^*

^*Corresponding author for this work

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

3 Citations (Scopus)

Abstract

This study presents a new microfluidic system for molecular diagnosis of human immunodeficiency virus (HIV) infection. In this study, magnetic beads conjugated with nucleotide probes specific to HIV were used to perform purification and enrichment of target DNA. Then an on-chip PCR module was used to perform amplification of four detection genes associated with HIV infection. Finally, an optical module was used to detect the amplified PCR products. Experimental results demonstrated that multiple HIV detection fragments could be amplified successfully and detected on-line using the developed microfluidic system in the HIV-infected cell line within 95 min, which is much faster than the existing methods. © 2012 IEEE.

Original language	English
Title of host publication	2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012
Pages	961-964
DOIs	https://doi.org/10.1109/MEMSYS.2012.6170346
Publication status	Published - 2012
Externally published	Yes
Event	25th IEEE International Conference on Micro Electro Mechanical Systems (MEMS 2012) - Paris, France Duration: 29 Jan 2012 → 2 Feb 2012 https://www.memsconferences.org/mems2012/

Publication series

Name	Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)	1084-6999

Conference

Conference	25th IEEE International Conference on Micro Electro Mechanical Systems (MEMS 2012)
Place	France
City	Paris
Period	29/01/12 → 2/02/12
Internet address	https://www.memsconferences.org/mems2012/

Bibliographical note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

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Wang, J.-H., Wang, C.-H., Ling, W.-S., Jheng, L., Wang, S.-W., & Lee, G.-B. (2012). Integrated microfluidic system for HIV detection. In 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012 (pp. 961-964). Article 6170346 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). https://doi.org/10.1109/MEMSYS.2012.6170346

@inproceedings{3f10cd3a18d142008b698690afaeb9a4,

title = "Integrated microfluidic system for HIV detection",

abstract = "This study presents a new microfluidic system for molecular diagnosis of human immunodeficiency virus (HIV) infection. In this study, magnetic beads conjugated with nucleotide probes specific to HIV were used to perform purification and enrichment of target DNA. Then an on-chip PCR module was used to perform amplification of four detection genes associated with HIV infection. Finally, an optical module was used to detect the amplified PCR products. Experimental results demonstrated that multiple HIV detection fragments could be amplified successfully and detected on-line using the developed microfluidic system in the HIV-infected cell line within 95 min, which is much faster than the existing methods. {\textcopyright} 2012 IEEE.",

author = "Jung-Hao Wang and Chih-Hung Wang and Wei-Shuo Ling and Lie Jheng and Shainn-Wei Wang and Gwo-Bin Lee",

note = "Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].; 25th IEEE International Conference on Micro Electro Mechanical Systems (MEMS 2012) ; Conference date: 29-01-2012 Through 02-02-2012",

year = "2012",

doi = "10.1109/MEMSYS.2012.6170346",

language = "English",

isbn = "9781467303248",

series = "Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)",

pages = "961--964",

booktitle = "2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012",

url = "https://www.memsconferences.org/mems2012/",

}

Wang, J-H, Wang, C-H, Ling, W-S, Jheng, L, Wang, S-W & Lee, G-B 2012, Integrated microfluidic system for HIV detection. in 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012., 6170346, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), pp. 961-964, 25th IEEE International Conference on Micro Electro Mechanical Systems (MEMS 2012), Paris, France, 29/01/12. https://doi.org/10.1109/MEMSYS.2012.6170346

Integrated microfluidic system for HIV detection. / Wang, Jung-Hao; Wang, Chih-Hung; Ling, Wei-Shuo et al.
2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012. 2012. p. 961-964 6170346 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

TY - GEN

T1 - Integrated microfluidic system for HIV detection

AU - Wang, Jung-Hao

AU - Wang, Chih-Hung

AU - Ling, Wei-Shuo

AU - Jheng, Lie

AU - Wang, Shainn-Wei

AU - Lee, Gwo-Bin

N1 - Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

PY - 2012

Y1 - 2012

N2 - This study presents a new microfluidic system for molecular diagnosis of human immunodeficiency virus (HIV) infection. In this study, magnetic beads conjugated with nucleotide probes specific to HIV were used to perform purification and enrichment of target DNA. Then an on-chip PCR module was used to perform amplification of four detection genes associated with HIV infection. Finally, an optical module was used to detect the amplified PCR products. Experimental results demonstrated that multiple HIV detection fragments could be amplified successfully and detected on-line using the developed microfluidic system in the HIV-infected cell line within 95 min, which is much faster than the existing methods. © 2012 IEEE.

AB - This study presents a new microfluidic system for molecular diagnosis of human immunodeficiency virus (HIV) infection. In this study, magnetic beads conjugated with nucleotide probes specific to HIV were used to perform purification and enrichment of target DNA. Then an on-chip PCR module was used to perform amplification of four detection genes associated with HIV infection. Finally, an optical module was used to detect the amplified PCR products. Experimental results demonstrated that multiple HIV detection fragments could be amplified successfully and detected on-line using the developed microfluidic system in the HIV-infected cell line within 95 min, which is much faster than the existing methods. © 2012 IEEE.

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U2 - 10.1109/MEMSYS.2012.6170346

DO - 10.1109/MEMSYS.2012.6170346

M3 - RGC 32 - Refereed conference paper (with host publication)

SN - 9781467303248

T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)

SP - 961

EP - 964

BT - 2012 IEEE 25th International Conference on Micro Electro Mechanical Systems, MEMS 2012

T2 - 25th IEEE International Conference on Micro Electro Mechanical Systems (MEMS 2012)

Y2 - 29 January 2012 through 2 February 2012

ER -

Integrated microfluidic system for HIV detection

Abstract

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Bibliographical note

UN SDGs

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