Integrated microfluidic sensors for amperometric detection of influenza virus using an aptamer-antibody assay

Yi-Ting Tseng; Kuangwen Hsieh; Gwo-Bin Lee

Integrated microfluidic sensors for amperometric detection of influenza virus using an aptamer-antibody assay

Yi-Ting Tseng
, Kuangwen Hsieh
, Gwo-Bin Lee

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

1 Citation (Scopus)

Abstract

Influenza H1N1 viruses have caused serious concerns over the past decade. As a result, development of automatic and compact devices is of great needs. Conventional approaches, however, suffer from several critical issues such as time-consuming and low specificity. In this work, we therefore present a new approach for amperometric detection of influenza A virus using a new aptamer-antibody assay, which could be automatically performed on an integrated microfluidic system. The limit of the detection was significantly improved due to the high affinity and specificity of the aptamer. Experimental data showed that the developed microfluidic system could detect influenza virus successfully.

Original language	English
Title of host publication	18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014
Publisher	Chemical and Biological Microsystems Society
Pages	2143-2145
ISBN (Print)	9780979806476
Publication status	Published - 2014
Externally published	Yes
Event	18th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2014) - San Antonio, United States Duration: 26 Oct 2014 → 30 Oct 2014

Publication series

Name	18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

Conference

Conference	18th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2014)
Place	United States
City	San Antonio
Period	26/10/14 → 30/10/14

Bibliographical note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Research Keywords

Amperometric detection
Aptamer
Influenza virus
Microfluidics

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Tseng, Y.-T., Hsieh, K., & Lee, G.-B. (2014). Integrated microfluidic sensors for amperometric detection of influenza virus using an aptamer-antibody assay. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 (pp. 2143-2145). (18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014). Chemical and Biological Microsystems Society.

Tseng, Yi-Ting ; Hsieh, Kuangwen ; Lee, Gwo-Bin. / Integrated microfluidic sensors for amperometric detection of influenza virus using an aptamer-antibody assay. 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, 2014. pp. 2143-2145 (18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014).

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abstract = "Influenza H1N1 viruses have caused serious concerns over the past decade. As a result, development of automatic and compact devices is of great needs. Conventional approaches, however, suffer from several critical issues such as time-consuming and low specificity. In this work, we therefore present a new approach for amperometric detection of influenza A virus using a new aptamer-antibody assay, which could be automatically performed on an integrated microfluidic system. The limit of the detection was significantly improved due to the high affinity and specificity of the aptamer. Experimental data showed that the developed microfluidic system could detect influenza virus successfully.",

keywords = "Amperometric detection, Aptamer, Influenza virus, Microfluidics",

author = "Yi-Ting Tseng and Kuangwen Hsieh and Gwo-Bin Lee",

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series = "18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014",

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Tseng, Y-T, Hsieh, K & Lee, G-B 2014, Integrated microfluidic sensors for amperometric detection of influenza virus using an aptamer-antibody assay. in 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, Chemical and Biological Microsystems Society, pp. 2143-2145, 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2014), San Antonio, Texas, United States, 26/10/14.

Integrated microfluidic sensors for amperometric detection of influenza virus using an aptamer-antibody assay. / Tseng, Yi-Ting; Hsieh, Kuangwen; Lee, Gwo-Bin.
18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society, 2014. p. 2143-2145 (18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014).

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

TY - GEN

T1 - Integrated microfluidic sensors for amperometric detection of influenza virus using an aptamer-antibody assay

AU - Tseng, Yi-Ting

AU - Hsieh, Kuangwen

AU - Lee, Gwo-Bin

N1 - Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

PY - 2014

Y1 - 2014

N2 - Influenza H1N1 viruses have caused serious concerns over the past decade. As a result, development of automatic and compact devices is of great needs. Conventional approaches, however, suffer from several critical issues such as time-consuming and low specificity. In this work, we therefore present a new approach for amperometric detection of influenza A virus using a new aptamer-antibody assay, which could be automatically performed on an integrated microfluidic system. The limit of the detection was significantly improved due to the high affinity and specificity of the aptamer. Experimental data showed that the developed microfluidic system could detect influenza virus successfully.

AB - Influenza H1N1 viruses have caused serious concerns over the past decade. As a result, development of automatic and compact devices is of great needs. Conventional approaches, however, suffer from several critical issues such as time-consuming and low specificity. In this work, we therefore present a new approach for amperometric detection of influenza A virus using a new aptamer-antibody assay, which could be automatically performed on an integrated microfluidic system. The limit of the detection was significantly improved due to the high affinity and specificity of the aptamer. Experimental data showed that the developed microfluidic system could detect influenza virus successfully.

KW - Amperometric detection

KW - Aptamer

KW - Influenza virus

KW - Microfluidics

UR - https://www.scopus.com/pages/publications/84941686341

UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-84941686341&origin=recordpage

M3 - RGC 32 - Refereed conference paper (with host publication)

SN - 9780979806476

T3 - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

SP - 2143

EP - 2145

BT - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014

PB - Chemical and Biological Microsystems Society

T2 - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences (MicroTAS 2014)

Y2 - 26 October 2014 through 30 October 2014

ER -

Tseng YT, Hsieh K, Lee GB. Integrated microfluidic sensors for amperometric detection of influenza virus using an aptamer-antibody assay. In 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014. Chemical and Biological Microsystems Society. 2014. p. 2143-2145. (18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014).

Integrated microfluidic sensors for amperometric detection of influenza virus using an aptamer-antibody assay

Abstract

Publication series

Conference

Bibliographical note

UN SDGs

Research Keywords

Other Access Options

Permanent Link

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