Integrated microfluidic loop-mediated-isothermal-amplification system for rapid diagnosis of aquaculture virus

Chih-Hung Wang; Kang-Yi Lien; Ting-Yu Wang; Tzong-Yueh Chen; Gwo-Bin Lee

Integrated microfluidic loop-mediated-isothermal-amplification system for rapid diagnosis of aquaculture virus

Chih-Hung Wang, Kang-Yi Lien, Ting-Yu Wang, Tzong-Yueh Chen, Gwo-Bin Lee

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

1 Citation (Scopus)

Abstract

The micro polymerase chain reaction (PCR) chip has been extensively reported for rapid clinical diagnosis recently. However, a series of heating and cooling processes may increase total reaction time and complexity of the control system. Besides, nucleic acid extraction, which is a critical procedure for molecular diagnosis, still remains an issue for most of the microfluidic systems. This study therefore, for the first time, reports a new automatic assay for ribonucleic acid (RNA) extraction and one-step reverse-transcription loop-mediated isothermal amplification (RT-LAMP) processes under a constant temperature for rapid detection of viruses from infected tissue samples by utilizing an integrated microfluidic system. Experimental data showed that the entire process for molecular diagnosis of an aquaculture virus from grouper tissues can be automatically performed within a short period of time and carried out with high sensitivity and specificity.

Original language	English
Title of host publication	14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Pages	812-814
Volume	2
Publication status	Published - 2010
Externally published	Yes
Event	14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 - Groningen, Netherlands Duration: 3 Oct 2010 → 7 Oct 2010

Publication series

Name	14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Volume	2

Conference

Conference	14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Place	Netherlands
City	Groningen
Period	3/10/10 → 7/10/10

Bibliographical note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 14 Life Below Water

Research Keywords

Loop mediated isothermal amplification
Microfluidics
Reverse transcription

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Wang, C.-H., Lien, K.-Y., Wang, T.-Y., Chen, T.-Y., & Lee, G.-B. (2010). Integrated microfluidic loop-mediated-isothermal-amplification system for rapid diagnosis of aquaculture virus. In 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 (Vol. 2, pp. 812-814). (14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010; Vol. 2).

Wang, Chih-Hung ; Lien, Kang-Yi ; Wang, Ting-Yu et al. / Integrated microfluidic loop-mediated-isothermal-amplification system for rapid diagnosis of aquaculture virus. 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. Vol. 2 2010. pp. 812-814 (14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010).

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title = "Integrated microfluidic loop-mediated-isothermal-amplification system for rapid diagnosis of aquaculture virus",

abstract = "The micro polymerase chain reaction (PCR) chip has been extensively reported for rapid clinical diagnosis recently. However, a series of heating and cooling processes may increase total reaction time and complexity of the control system. Besides, nucleic acid extraction, which is a critical procedure for molecular diagnosis, still remains an issue for most of the microfluidic systems. This study therefore, for the first time, reports a new automatic assay for ribonucleic acid (RNA) extraction and one-step reverse-transcription loop-mediated isothermal amplification (RT-LAMP) processes under a constant temperature for rapid detection of viruses from infected tissue samples by utilizing an integrated microfluidic system. Experimental data showed that the entire process for molecular diagnosis of an aquaculture virus from grouper tissues can be automatically performed within a short period of time and carried out with high sensitivity and specificity.",

keywords = "Loop mediated isothermal amplification, Microfluidics, Reverse transcription",

author = "Chih-Hung Wang and Kang-Yi Lien and Ting-Yu Wang and Tzong-Yueh Chen and Gwo-Bin Lee",

note = "Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].; 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 ; Conference date: 03-10-2010 Through 07-10-2010",

year = "2010",

language = "English",

isbn = "9781618390622",

volume = "2",

series = "14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010",

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booktitle = "14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010",

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Wang, C-H, Lien, K-Y, Wang, T-Y, Chen, T-Y & Lee, G-B 2010, Integrated microfluidic loop-mediated-isothermal-amplification system for rapid diagnosis of aquaculture virus. in 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. vol. 2, 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010, vol. 2, pp. 812-814, 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010, Groningen, Netherlands, 3/10/10.

Integrated microfluidic loop-mediated-isothermal-amplification system for rapid diagnosis of aquaculture virus. / Wang, Chih-Hung; Lien, Kang-Yi; Wang, Ting-Yu et al.
14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. Vol. 2 2010. p. 812-814 (14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010; Vol. 2).

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

TY - GEN

T1 - Integrated microfluidic loop-mediated-isothermal-amplification system for rapid diagnosis of aquaculture virus

AU - Wang, Chih-Hung

AU - Lien, Kang-Yi

AU - Wang, Ting-Yu

AU - Chen, Tzong-Yueh

AU - Lee, Gwo-Bin

N1 - Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

PY - 2010

Y1 - 2010

N2 - The micro polymerase chain reaction (PCR) chip has been extensively reported for rapid clinical diagnosis recently. However, a series of heating and cooling processes may increase total reaction time and complexity of the control system. Besides, nucleic acid extraction, which is a critical procedure for molecular diagnosis, still remains an issue for most of the microfluidic systems. This study therefore, for the first time, reports a new automatic assay for ribonucleic acid (RNA) extraction and one-step reverse-transcription loop-mediated isothermal amplification (RT-LAMP) processes under a constant temperature for rapid detection of viruses from infected tissue samples by utilizing an integrated microfluidic system. Experimental data showed that the entire process for molecular diagnosis of an aquaculture virus from grouper tissues can be automatically performed within a short period of time and carried out with high sensitivity and specificity.

AB - The micro polymerase chain reaction (PCR) chip has been extensively reported for rapid clinical diagnosis recently. However, a series of heating and cooling processes may increase total reaction time and complexity of the control system. Besides, nucleic acid extraction, which is a critical procedure for molecular diagnosis, still remains an issue for most of the microfluidic systems. This study therefore, for the first time, reports a new automatic assay for ribonucleic acid (RNA) extraction and one-step reverse-transcription loop-mediated isothermal amplification (RT-LAMP) processes under a constant temperature for rapid detection of viruses from infected tissue samples by utilizing an integrated microfluidic system. Experimental data showed that the entire process for molecular diagnosis of an aquaculture virus from grouper tissues can be automatically performed within a short period of time and carried out with high sensitivity and specificity.

KW - Loop mediated isothermal amplification

KW - Microfluidics

KW - Reverse transcription

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UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-84884391573&origin=recordpage

M3 - RGC 32 - Refereed conference paper (with host publication)

SN - 9781618390622

VL - 2

T3 - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010

SP - 812

EP - 814

BT - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010

T2 - 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010

Y2 - 3 October 2010 through 7 October 2010

ER -

Wang CH, Lien KY, Wang TY, Chen TY, Lee GB. Integrated microfluidic loop-mediated-isothermal-amplification system for rapid diagnosis of aquaculture virus. In 14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010. Vol. 2. 2010. p. 812-814. (14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010).

Integrated microfluidic loop-mediated-isothermal-amplification system for rapid diagnosis of aquaculture virus

Abstract

Publication series

Conference

Bibliographical note

UN SDGs

Research Keywords

Other Access Options

Permanent Link

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