Integrated microfluidic chip for measuring T helper cells

Jung-Hao Wang; Chih-Hung Wang; Chun-Che Lin; Gwo-Bin Lee

doi:10.1109/MEMSYS.2010.5442366

Integrated microfluidic chip for measuring T helper cells

Jung-Hao Wang
, Chih-Hung Wang
, Chun-Che Lin
, Gwo-Bin Lee

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

1 Citation (Scopus)

Abstract

This paper reports a new microfluidic chip capable of measuring and counting CD3/CD4 T helper cells. A T-cell count was essential for diagnosis of immunodeficiency and lymphocytic diseases. To achieve the measurement and counting of T helper cells, a new microfluidic device comprising a micro incubation and a micro flow cytometer module was presented in this study for sample pretreatment, measuring, and counting. First, the CD3/CD4 T helper cells and anti-human CD3/CD4 antibodies conjugated fluorescent proteins were co-incubated in the micro incubation. When the T helper cells flew through the micro flow cytometer, the intensity of fluorescent signals was then detected. The T helper cells would be counted unless both signals of anti-human CD3 and CD4 antibodies were induced at the same time. Thus, the specificity of T helper cells was measured to be 0.96. The developed microfluidic chip may provide a promising tool for fast monitoring of immunodeficiency and lymphocytic diseases. ©2010 IEEE.

Original language	English
Title of host publication	MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest
Pages	955-958
DOIs	https://doi.org/10.1109/MEMSYS.2010.5442366
Publication status	Published - 2010
Externally published	Yes
Event	23rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2010 - , Hong Kong, China Duration: 24 Jan 2010 → 28 Jan 2010 https://ieeexplore.ieee.org/xpl/conhome/5438531/proceeding

Publication series

Name	Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)	1084-6999

Conference

Conference	23rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2010
Place	Hong Kong, China
Period	24/01/10 → 28/01/10
Internet address	https://ieeexplore.ieee.org/xpl/conhome/5438531/proceeding

Bibliographical note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

Access Output

10.1109/MEMSYS.2010.5442366

Other Access Options

Check CityUHK Library

Permanent Link

Right click to copy link

Cite this

Wang, J.-H., Wang, C.-H., Lin, C.-C., & Lee, G.-B. (2010). Integrated microfluidic chip for measuring T helper cells. In MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest (pp. 955-958). Article 5442366 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). https://doi.org/10.1109/MEMSYS.2010.5442366

@inproceedings{70a842e919c74339962b7583c7aa3e46,

title = "Integrated microfluidic chip for measuring T helper cells",

abstract = "This paper reports a new microfluidic chip capable of measuring and counting CD3/CD4 T helper cells. A T-cell count was essential for diagnosis of immunodeficiency and lymphocytic diseases. To achieve the measurement and counting of T helper cells, a new microfluidic device comprising a micro incubation and a micro flow cytometer module was presented in this study for sample pretreatment, measuring, and counting. First, the CD3/CD4 T helper cells and anti-human CD3/CD4 antibodies conjugated fluorescent proteins were co-incubated in the micro incubation. When the T helper cells flew through the micro flow cytometer, the intensity of fluorescent signals was then detected. The T helper cells would be counted unless both signals of anti-human CD3 and CD4 antibodies were induced at the same time. Thus, the specificity of T helper cells was measured to be 0.96. The developed microfluidic chip may provide a promising tool for fast monitoring of immunodeficiency and lymphocytic diseases. {\textcopyright}2010 IEEE.",

author = "Jung-Hao Wang and Chih-Hung Wang and Chun-Che Lin and Gwo-Bin Lee",

note = "Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].; 23rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2010 ; Conference date: 24-01-2010 Through 28-01-2010",

year = "2010",

doi = "10.1109/MEMSYS.2010.5442366",

language = "English",

isbn = "9781424457649",

series = "Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)",

pages = "955--958",

booktitle = "MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest",

url = "https://ieeexplore.ieee.org/xpl/conhome/5438531/proceeding",

}

Wang, J-H, Wang, C-H, Lin, C-C & Lee, G-B 2010, Integrated microfluidic chip for measuring T helper cells. in MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest., 5442366, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), pp. 955-958, 23rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2010, Hong Kong, China, 24/01/10. https://doi.org/10.1109/MEMSYS.2010.5442366

Integrated microfluidic chip for measuring T helper cells. / Wang, Jung-Hao; Wang, Chih-Hung; Lin, Chun-Che et al.
MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest. 2010. p. 955-958 5442366 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

TY - GEN

T1 - Integrated microfluidic chip for measuring T helper cells

AU - Wang, Jung-Hao

AU - Wang, Chih-Hung

AU - Lin, Chun-Che

AU - Lee, Gwo-Bin

N1 - Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].

PY - 2010

Y1 - 2010

N2 - This paper reports a new microfluidic chip capable of measuring and counting CD3/CD4 T helper cells. A T-cell count was essential for diagnosis of immunodeficiency and lymphocytic diseases. To achieve the measurement and counting of T helper cells, a new microfluidic device comprising a micro incubation and a micro flow cytometer module was presented in this study for sample pretreatment, measuring, and counting. First, the CD3/CD4 T helper cells and anti-human CD3/CD4 antibodies conjugated fluorescent proteins were co-incubated in the micro incubation. When the T helper cells flew through the micro flow cytometer, the intensity of fluorescent signals was then detected. The T helper cells would be counted unless both signals of anti-human CD3 and CD4 antibodies were induced at the same time. Thus, the specificity of T helper cells was measured to be 0.96. The developed microfluidic chip may provide a promising tool for fast monitoring of immunodeficiency and lymphocytic diseases. ©2010 IEEE.

AB - This paper reports a new microfluidic chip capable of measuring and counting CD3/CD4 T helper cells. A T-cell count was essential for diagnosis of immunodeficiency and lymphocytic diseases. To achieve the measurement and counting of T helper cells, a new microfluidic device comprising a micro incubation and a micro flow cytometer module was presented in this study for sample pretreatment, measuring, and counting. First, the CD3/CD4 T helper cells and anti-human CD3/CD4 antibodies conjugated fluorescent proteins were co-incubated in the micro incubation. When the T helper cells flew through the micro flow cytometer, the intensity of fluorescent signals was then detected. The T helper cells would be counted unless both signals of anti-human CD3 and CD4 antibodies were induced at the same time. Thus, the specificity of T helper cells was measured to be 0.96. The developed microfluidic chip may provide a promising tool for fast monitoring of immunodeficiency and lymphocytic diseases. ©2010 IEEE.

UR - https://www.scopus.com/pages/publications/77952784492

UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-77952784492&origin=recordpage

U2 - 10.1109/MEMSYS.2010.5442366

DO - 10.1109/MEMSYS.2010.5442366

M3 - RGC 32 - Refereed conference paper (with host publication)

SN - 9781424457649

T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)

SP - 955

EP - 958

BT - MEMS 2010 - The 23rd IEEE International Conference on Micro Electro Mechanical Systems, Technical Digest

T2 - 23rd IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2010

Y2 - 24 January 2010 through 28 January 2010

ER -

Integrated microfluidic chip for measuring T helper cells

Abstract

Publication series

Conference

Bibliographical note

Access Output

Other Access Options

Permanent Link

Other Files and Links

Fingerprint

Cite this