Integrated Analytical System for Clinical Single-Cell Analysis

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

4 Scopus Citations
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Author(s)

  • Hannah M. Peterson
  • Yoshiko Iwamoto
  • Juhyun Oh
  • Jonathan C. T. Carlson
  • Hakho Lee
  • Hyungsoon Im
  • Ralph Weissleder

Detail(s)

Original languageEnglish
Article number2200415
Number of pages12
Journal / PublicationAdvanced Science
Volume9
Issue number20
Online published4 May 2022
Publication statusPublished - 15 Jul 2022
Externally publishedYes

Link(s)

Abstract

High-dimensional analyses of cancers can potentially be used to better define cancer subtypes, analyze the complex tumor microenvironment, and perform cancer cell pathway analyses for drug trials. Unfortunately, integrated systems that allow such analyses in serial fine needle aspirates within a day or at point-of-care currently do not exist. To achieve this, an integrated immunofluorescence single-cell analyzer (i2SCAN) for deep profiling of directly harvested cells is developed. By combining a novel cellular imaging system, highly cyclable bioorthogonal FAST antibody panels, and integrated computational analysis, it is shown that same-day analysis is possible in thousands of harvested cells. It is demonstrated that the i2SCAN approach allows comprehensive analysis of breast cancer samples obtained by fine needle aspiration or core tissues. The method is a rapid, robust, and low-cost solution to high-dimensional analysis of scant clinical specimens.

Research Area(s)

  • biopsy, cancer, deep profiling, diagnostic

Citation Format(s)

Integrated Analytical System for Clinical Single-Cell Analysis. / Peterson, Hannah M.; Chin, Lip Ket; Iwamoto, Yoshiko et al.
In: Advanced Science, Vol. 9, No. 20, 2200415, 15.07.2022.

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

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