Insulin detection based on a PDMS microfluidic system

Zhikun Zhan; Ping Yao; Zaili Dong; Steve Tung; Jacob Hohnbaum; Balaji Srinivasan; Wen J. Li

doi:10.1109/NANOMED.2010.5749815

Insulin detection based on a PDMS microfluidic system

Zhikun Zhan, Ping Yao, Zaili Dong, Steve Tung, Jacob Hohnbaum, Balaji Srinivasan, Wen J. Li

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

4 Citations (Scopus)

Abstract

An integrated polydimethysiloxane (PDMS) microfluidic system which is composed of two pneumatic micropumps and one micromixer is developed for high-accuracy detection of insulin. The detection method is based on coupling the highly specific technique of 'double-antibody sandwich immunoassay' with the sensitive chemiluminescence of Luminol-Hydrogen Peroxide (H₂O ₂). The immune reactions and other related processes are carried out in the microfluidic system semi automatically. Sample transportation in the microfluidic system is accomplished by two pneumatic PDMS micropumps. Chemiluminescent measurement is conducted in a separate PDMS micromixer using a double-channel syringe pump to inject reagents. Light emitting from this mixer is detected by a highly sensitive photometer when the chemiluminescent regents flow through the mixer chamber. The results indicate that at an actuation pressure of 10psi, a mixer actuation frequency of 5Hz, and an injecting flow rate of 0.5 ml/min, the detection limit of the microfluidic system for insulin is about 10^-10 M. © 2010 IEEE.

Original language	English
Title of host publication	2010 IEEE International Conference on Nano/Molecular Medicine and Engineering, IEEE NANOMED 2010
Pages	112-116
DOIs	https://doi.org/10.1109/NANOMED.2010.5749815
Publication status	Published - 2010
Externally published	Yes
Event	4th IEEE International Conference on Nano/Molecular Medicine and Engineering (IEEE NANOMED 2010) - Hong Kong/Macau, China Duration: 5 Dec 2010 → 9 Dec 2010

Conference

Conference	4th IEEE International Conference on Nano/Molecular Medicine and Engineering (IEEE NANOMED 2010)
Country/Territory	China
City	Hong Kong/Macau
Period	5/12/10 → 9/12/10

Research Keywords

insulin
microfluidic system
micromixer chemiluminescence immunoassay
micropump

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10.1109/NANOMED.2010.5749815

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@inproceedings{64becce047764300937af532c1ff882b,

title = "Insulin detection based on a PDMS microfluidic system",

abstract = "An integrated polydimethysiloxane (PDMS) microfluidic system which is composed of two pneumatic micropumps and one micromixer is developed for high-accuracy detection of insulin. The detection method is based on coupling the highly specific technique of 'double-antibody sandwich immunoassay' with the sensitive chemiluminescence of Luminol-Hydrogen Peroxide (H2O 2). The immune reactions and other related processes are carried out in the microfluidic system semi automatically. Sample transportation in the microfluidic system is accomplished by two pneumatic PDMS micropumps. Chemiluminescent measurement is conducted in a separate PDMS micromixer using a double-channel syringe pump to inject reagents. Light emitting from this mixer is detected by a highly sensitive photometer when the chemiluminescent regents flow through the mixer chamber. The results indicate that at an actuation pressure of 10psi, a mixer actuation frequency of 5Hz, and an injecting flow rate of 0.5 ml/min, the detection limit of the microfluidic system for insulin is about 10-10 M. {\textcopyright} 2010 IEEE.",

keywords = "insulin, microfluidic system, micromixer chemiluminescence immunoassay, micropump",

author = "Zhikun Zhan and Ping Yao and Zaili Dong and Steve Tung and Jacob Hohnbaum and Balaji Srinivasan and Li, {Wen J.}",

year = "2010",

doi = "10.1109/NANOMED.2010.5749815",

language = "English",

isbn = "9781612841533",

pages = "112--116",

booktitle = "2010 IEEE International Conference on Nano/Molecular Medicine and Engineering, IEEE NANOMED 2010",

note = "4th IEEE International Conference on Nano/Molecular Medicine and Engineering (IEEE NANOMED 2010) ; Conference date: 05-12-2010 Through 09-12-2010",

}

Zhan, Z, Yao, P, Dong, Z, Tung, S, Hohnbaum, J, Srinivasan, B & Li, WJ 2010, Insulin detection based on a PDMS microfluidic system. in 2010 IEEE International Conference on Nano/Molecular Medicine and Engineering, IEEE NANOMED 2010., 5749815, pp. 112-116, 4th IEEE International Conference on Nano/Molecular Medicine and Engineering (IEEE NANOMED 2010), Hong Kong/Macau, China, 5/12/10. https://doi.org/10.1109/NANOMED.2010.5749815

Insulin detection based on a PDMS microfluidic system. / Zhan, Zhikun; Yao, Ping; Dong, Zaili et al.
2010 IEEE International Conference on Nano/Molecular Medicine and Engineering, IEEE NANOMED 2010. 2010. p. 112-116 5749815.

Research output: Chapters, Conference Papers, Creative and Literary Works › RGC 32 - Refereed conference paper (with host publication) › peer-review

TY - GEN

T1 - Insulin detection based on a PDMS microfluidic system

AU - Zhan, Zhikun

AU - Yao, Ping

AU - Dong, Zaili

AU - Tung, Steve

AU - Hohnbaum, Jacob

AU - Srinivasan, Balaji

AU - Li, Wen J.

PY - 2010

Y1 - 2010

N2 - An integrated polydimethysiloxane (PDMS) microfluidic system which is composed of two pneumatic micropumps and one micromixer is developed for high-accuracy detection of insulin. The detection method is based on coupling the highly specific technique of 'double-antibody sandwich immunoassay' with the sensitive chemiluminescence of Luminol-Hydrogen Peroxide (H2O 2). The immune reactions and other related processes are carried out in the microfluidic system semi automatically. Sample transportation in the microfluidic system is accomplished by two pneumatic PDMS micropumps. Chemiluminescent measurement is conducted in a separate PDMS micromixer using a double-channel syringe pump to inject reagents. Light emitting from this mixer is detected by a highly sensitive photometer when the chemiluminescent regents flow through the mixer chamber. The results indicate that at an actuation pressure of 10psi, a mixer actuation frequency of 5Hz, and an injecting flow rate of 0.5 ml/min, the detection limit of the microfluidic system for insulin is about 10-10 M. © 2010 IEEE.

AB - An integrated polydimethysiloxane (PDMS) microfluidic system which is composed of two pneumatic micropumps and one micromixer is developed for high-accuracy detection of insulin. The detection method is based on coupling the highly specific technique of 'double-antibody sandwich immunoassay' with the sensitive chemiluminescence of Luminol-Hydrogen Peroxide (H2O 2). The immune reactions and other related processes are carried out in the microfluidic system semi automatically. Sample transportation in the microfluidic system is accomplished by two pneumatic PDMS micropumps. Chemiluminescent measurement is conducted in a separate PDMS micromixer using a double-channel syringe pump to inject reagents. Light emitting from this mixer is detected by a highly sensitive photometer when the chemiluminescent regents flow through the mixer chamber. The results indicate that at an actuation pressure of 10psi, a mixer actuation frequency of 5Hz, and an injecting flow rate of 0.5 ml/min, the detection limit of the microfluidic system for insulin is about 10-10 M. © 2010 IEEE.

KW - insulin

KW - microfluidic system

KW - micromixer chemiluminescence immunoassay

KW - micropump

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U2 - 10.1109/NANOMED.2010.5749815

DO - 10.1109/NANOMED.2010.5749815

M3 - RGC 32 - Refereed conference paper (with host publication)

SN - 9781612841533

SP - 112

EP - 116

BT - 2010 IEEE International Conference on Nano/Molecular Medicine and Engineering, IEEE NANOMED 2010

T2 - 4th IEEE International Conference on Nano/Molecular Medicine and Engineering (IEEE NANOMED 2010)

Y2 - 5 December 2010 through 9 December 2010

ER -

Insulin detection based on a PDMS microfluidic system

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