Inhibition of telomerase activity and bcl-2 expression in berbamine-induced apoptosis in HL-60 cells
Research output: Journal Publications and Reviews (RGC: 21, 22, 62) › 21_Publication in refereed journal › peer-review
Author(s)
Detail(s)
| Original language | English |
|---|---|
| Pages (from-to) | 596-600 |
| Journal / Publication | Planta Medica |
| Volume | 68 |
| Issue number | 7 |
| Publication status | Published - 2002 |
| Externally published | Yes |
Link(s)
Abstract
The present study was aimed to investigate the effect of telomerase activity in berbamine-induced apoptosis and the regulation of B cell leukemia/lymphoma 2 (bcl-2) gene expression in human leukemia HL-60 cells. Apoptosis of HL-60 cells was induced by berbamine (10 μM) for 3, 6, 12 and 24 h. Apoptosis and bcl-2 were determined by flow cytometry analysis. A polymerase chain reaction-based telomeric repeat amplification protocol assay was used to detect the telomerase activity. Berbamine induced growth arrest and apoptotic cell death in HL-60 cells. The telomerase activity was inhibited in a time-dependent manner during the berbamine-induced apoptosis of HL-60 cells, and the expression of bcl-2 was progressively down-regulated by berbamine. Inhibition of the telomerase activity of HL-60 cells was closely related to the berbamine-induced apoptosis. The present results indicate that inhibition of telomerase and reduced bcl-2 gene expression may play a role in the berbamine-induced apoptosis of HL-60 cells.
Research Area(s)
- Apoptosis, Bcl-2 gene, Berbamine, HL-60 cell, Menispermum dauricum, Telomerase
Bibliographic Note
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Citation Format(s)
Inhibition of telomerase activity and bcl-2 expression in berbamine-induced apoptosis in HL-60 cells. / Ji, Zhao-Ning; Ye, Wen-Cai; Liu, Guo-Qing et al.
In: Planta Medica, Vol. 68, No. 7, 2002, p. 596-600.
In: Planta Medica, Vol. 68, No. 7, 2002, p. 596-600.
Research output: Journal Publications and Reviews (RGC: 21, 22, 62) › 21_Publication in refereed journal › peer-review
