Inhibition of telomerase activity and bcl-2 expression in berbamine-induced apoptosis in HL-60 cells

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review

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Author(s)

  • Zhao-Ning Ji
  • Wen-Cai Ye
  • Guo-Qing Liu
  • Yu Huang

Detail(s)

Original languageEnglish
Pages (from-to)596-600
Journal / PublicationPlanta Medica
Volume68
Issue number7
Publication statusPublished - 2002
Externally publishedYes

Abstract

The present study was aimed to investigate the effect of telomerase activity in berbamine-induced apoptosis and the regulation of B cell leukemia/lymphoma 2 (bcl-2) gene expression in human leukemia HL-60 cells. Apoptosis of HL-60 cells was induced by berbamine (10 μM) for 3, 6, 12 and 24 h. Apoptosis and bcl-2 were determined by flow cytometry analysis. A polymerase chain reaction-based telomeric repeat amplification protocol assay was used to detect the telomerase activity. Berbamine induced growth arrest and apoptotic cell death in HL-60 cells. The telomerase activity was inhibited in a time-dependent manner during the berbamine-induced apoptosis of HL-60 cells, and the expression of bcl-2 was progressively down-regulated by berbamine. Inhibition of the telomerase activity of HL-60 cells was closely related to the berbamine-induced apoptosis. The present results indicate that inhibition of telomerase and reduced bcl-2 gene expression may play a role in the berbamine-induced apoptosis of HL-60 cells.

Research Area(s)

  • Apoptosis, Bcl-2 gene, Berbamine, HL-60 cell, Menispermum dauricum, Telomerase

Bibliographic Note

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Citation Format(s)

Inhibition of telomerase activity and bcl-2 expression in berbamine-induced apoptosis in HL-60 cells. / Ji, Zhao-Ning; Ye, Wen-Cai; Liu, Guo-Qing et al.
In: Planta Medica, Vol. 68, No. 7, 2002, p. 596-600.

Research output: Journal Publications and ReviewsRGC 21 - Publication in refereed journalpeer-review