Inhibition of hepatitis B virus replication by stably expressed shRNA

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

58 Scopus Citations
View graph of relations

Author(s)

  • Ying Chen
  • Dan Du
  • Jun Wu
  • Chun-Pong Chan
  • Yuequi Tan
  • Hsiang-Fu Kung

Detail(s)

Original languageEnglish
Pages (from-to)398-404
Journal / PublicationBiochemical and Biophysical Research Communications
Volume311
Issue number2
Publication statusPublished - 14 Nov 2003
Externally publishedYes

Abstract

The major challenges for anti-hepatitis B Virus (HBV) therapy are the low efficacy of current drugs and the occurrence of drug resistant HBV mutations. A drug with new target sites or independent metabolic pathways may overcome these shortcomings. Small interfering RNA (siRNA) offers the possibility of developing a new anti-HBV therapy. Here we describe the almost complete inhibition of HBV replication by stably expressed 21-mer short hairpin RNAs (shRNA). Besides the conventional targets on HBV reverse-transcriptase, we also systemically targeted other sites of pregenomic RNA, including direct repeat (DR) elements, S, core, and X gene. Our results indicated that shRNAs can serve as efficient alternative anti-HBV agents. They can also be used in combination with chemotherapy, because they showed better effects on the inhibition of HBV replication due to different mechanisms of drug actions. © 2003 Elsevier Inc. All rights reserved.

Research Area(s)

  • Anti-HBV, Hepatitis B Virus, RNA interference, shRNA, U6 promoter

Citation Format(s)

Inhibition of hepatitis B virus replication by stably expressed shRNA. / Chen, Ying; Du, Dan; Wu, Jun; Chan, Chun-Pong; Tan, Yuequi; Kung, Hsiang-Fu; He, Ming-Liang.

In: Biochemical and Biophysical Research Communications, Vol. 311, No. 2, 14.11.2003, p. 398-404.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review