Inhibition of hepatitis B virus replication by stably expressed shRNA

Ying Chen; Dan Du; Jun Wu; Chun-Pong Chan; Yuequi Tan; Hsiang-Fu Kung; Ming-Liang He

doi:10.1016/j.bbrc.2003.10.009

Author(s)

Ying Chen
Dan Du
Jun Wu
Chun-Pong Chan
Yuequi Tan
Hsiang-Fu Kung

Detail(s)

Original language	English
Pages (from-to)	398-404
Journal / Publication	Biochemical and Biophysical Research Communications
Volume	311
Issue number	2
Publication status	Published - 14 Nov 2003
Externally published	Yes

Link(s)

DOI	DOI https://doi.org/10.1016/j.bbrc.2003.10.009 Final Published version
	Check@CityULib
Link to Scopus	https://www.scopus.com/record/display.uri?eid=2-s2.0-0242331285&origin=recordpage
Permanent Link	https://scholars.cityu.edu.hk/en/publications/publication(f5b2476f-63b1-4c81-9c91-fd72c5835975).html

Abstract

The major challenges for anti-hepatitis B Virus (HBV) therapy are the low efficacy of current drugs and the occurrence of drug resistant HBV mutations. A drug with new target sites or independent metabolic pathways may overcome these shortcomings. Small interfering RNA (siRNA) offers the possibility of developing a new anti-HBV therapy. Here we describe the almost complete inhibition of HBV replication by stably expressed 21-mer short hairpin RNAs (shRNA). Besides the conventional targets on HBV reverse-transcriptase, we also systemically targeted other sites of pregenomic RNA, including direct repeat (DR) elements, S, core, and X gene. Our results indicated that shRNAs can serve as efficient alternative anti-HBV agents. They can also be used in combination with chemotherapy, because they showed better effects on the inhibition of HBV replication due to different mechanisms of drug actions. © 2003 Elsevier Inc. All rights reserved.

Research Area(s)

Anti-HBV, Hepatitis B Virus, RNA interference, shRNA, U6 promoter

SDG 3 - Good Health and Well-being

Citation Format(s)

[ RIS ] [ BibTeX ]

Inhibition of hepatitis B virus replication by stably expressed shRNA. / Chen, Ying; Du, Dan; Wu, Jun et al.
In: Biochemical and Biophysical Research Communications, Vol. 311, No. 2, 14.11.2003, p. 398-404.

Research output: Journal Publications and Reviews › RGC 21 - Publication in refereed journal › peer-review

Chen, Y, Du, D, Wu, J, Chan, C-P, Tan, Y, Kung, H-F & He, M-L 2003, 'Inhibition of hepatitis B virus replication by stably expressed shRNA', Biochemical and Biophysical Research Communications, vol. 311, no. 2, pp. 398-404. https://doi.org/10.1016/j.bbrc.2003.10.009

Chen, Y., Du, D., Wu, J., Chan, C.-P., Tan, Y., Kung, H.-F., & He, M.-L. (2003). Inhibition of hepatitis B virus replication by stably expressed shRNA. Biochemical and Biophysical Research Communications, 311(2), 398-404. https://doi.org/10.1016/j.bbrc.2003.10.009

Chen Y, Du D, Wu J, Chan CP, Tan Y, Kung HF et al. Inhibition of hepatitis B virus replication by stably expressed shRNA. Biochemical and Biophysical Research Communications. 2003 Nov 14;311(2):398-404. doi: 10.1016/j.bbrc.2003.10.009

Chen, Ying ; Du, Dan ; Wu, Jun et al. / Inhibition of hepatitis B virus replication by stably expressed shRNA. In: Biochemical and Biophysical Research Communications. 2003 ; Vol. 311, No. 2. pp. 398-404.