Incubation and activity determination of lactate dehydrogenase isoenzymes on microfluidic chip
Research output: Journal Publications and Reviews (RGC: 21, 22, 62) › 21_Publication in refereed journal › peer-review
Author(s)
Detail(s)
| Original language | English |
|---|---|
| Pages (from-to) | 1223-1226 |
| Journal / Publication | Gaodeng Xuexiao Huaxue Xuebao/Chemical Journal of Chinese Universities |
| Volume | 27 |
| Issue number | 7 |
| Publication status | Published - Jul 2006 |
| Externally published | Yes |
Link(s)
Abstract
A novel incubation and activity determination method of lactate dehydrogenase isoenzymes was established. Based on the on-chip electrophoretic separation theory the analyzer was integrated with a temperature-controlling system. The assay program of the enzymes including injection of samples, incubation and separation was highly performed in the microchannel. A system for high sensitivity fluorescence detection was developed. A 360 nm light source was adopted to excite the fluorescence and the 460 nm signature was finally selectively gathered. The efficient separation of different LDH fractions indicates the potential of microfluidic devices for isoenzymes assay and the detection limit reached 0.5 U/L.
Research Area(s)
- Activity determination, Lactate dehydrogenase isoenzymes, Microfluidic chip, Temperature-controlling
Citation Format(s)
Incubation and activity determination of lactate dehydrogenase isoenzymes on microfluidic chip. / Liu, Jing; Zhuang, Gui-Sheng; Jia, Chun-Ping et al.
In: Gaodeng Xuexiao Huaxue Xuebao/Chemical Journal of Chinese Universities, Vol. 27, No. 7, 07.2006, p. 1223-1226.Research output: Journal Publications and Reviews (RGC: 21, 22, 62) › 21_Publication in refereed journal › peer-review
