Increasing prevalence of extended-spectrum cephalosporin-resistant Escherichia coli in food animals and the diversity of CTX-M genotypes during 2003-2012

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Lili Rao
  • Luchao Lv
  • Zhenling Zeng
  • Dandan He
  • Xiaojie Chen
  • Congming Wu
  • Yang Wang
  • Tong Yang
  • Peng Wu
  • Yahong Liu
  • Jian-Hua Liu

Detail(s)

Original languageEnglish
Pages (from-to)534-541
Journal / PublicationVeterinary Microbiology
Volume172
Issue number3-4
Online published22 Jun 2014
Publication statusPublished - 27 Aug 2014
Externally publishedYes

Abstract

The aim of this study was to investigate the trends and the diversity of CTX-M types of extended-spectrum β-lactamase (ESBL) in Escherichia coli isolated from food animals in China over a ten-year period. From 2003 to 2012, 2815 E. coli isolates collected from diseased animals (chickens, pigs, and waterfowl) were screened for the prevalence of CTX-M genes. CTX-M-positive isolates were tested for their susceptibilities to 10 antimicrobial agents and the clonal relationship of CTX-M-producing E. coli isolates was also assessed. Overall, 677 (20.1%) of the 2815 E. coli isolates carried CTX-M genes. Eighteen different types of CTX-M ESBLs were identified, with CTX-M-14, CTX-M-55, and CTX-M-65 being the most dominant genotypes. The occurrence of CTX-M-producing E. coli increased significantly from 5.7% in 2003-2005 to 35.3% in 2009-2012 (p< 0.0001). High genetic heterogeneities were observed in the CTX-M-producing E. coli isolates. Most CTX-M-producing strains were also resistant to other classes of antimicrobials. Compared to isolates carrying CTX-M-9 subgroup of ESBLs, isolates carrying CTX-M-1 subgroup ESBLs showed significantly higher resistance rates to ceftazidime, amikacin, and fosfomycin (p< 0.01). The study reported the dramatic increase of CTX-M ESBLs in E. coli isolated from animals overtime in China. The increasing incidence of CTX-M-55 with high hydrolytic activity against ceftazidime and the widely spread co-resistance in CTX-M-producing isolates alarm the serious antimicrobial resistance situation in China and highlight the need for urgent control strategies to limit the dissemination of those resistant genes in China. 

Research Area(s)

  • Animal reservoirs, CTX-M, Escherichia coli, Food animals, Fosfomycin

Citation Format(s)

Increasing prevalence of extended-spectrum cephalosporin-resistant Escherichia coli in food animals and the diversity of CTX-M genotypes during 2003-2012. / Rao, Lili; Lv, Luchao; Zeng, Zhenling; Chen, Sheng; He, Dandan; Chen, Xiaojie; Wu, Congming; Wang, Yang; Yang, Tong; Wu, Peng; Liu, Yahong; Liu, Jian-Hua.

In: Veterinary Microbiology, Vol. 172, No. 3-4, 27.08.2014, p. 534-541.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review