In vivo retinotopic mapping of superior colliculus using manganese-enhanced magnetic resonance imaging

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Kevin C. Chan
  • Jiang Li
  • Phillis Kau
  • Iris Y. Zhou
  • Matthew M. Cheung
  • Jian Yang
  • Kwok-fai So
  • Ed X. Wu

Detail(s)

Original languageEnglish
Pages (from-to)389-395
Journal / PublicationNeuroImage
Volume54
Issue number1
Publication statusPublished - 1 Jan 2011
Externally publishedYes

Abstract

The superior colliculus (SC) is a dome-shaped subcortical laminar structure in the mammalian midbrain, whose superficial layers receive visual information from the retina in a topological order. Despite the increasing number of studies investigating retinotopic projection in visual brain development and disorders, in vivo, high-resolution 3D mapping of topographic organization in the subcortical visual nuclei has not yet been available. This study explores the capability of 3D manganese-enhanced MRI (MEMRI) at 200μm isotropic resolution for in vivo retinotopic mapping of the rat SC upon partial transection of the intraorbital optic nerve. One day after intravitreal Mn2+ injection into both eyes, animals with partial transection at the right superior intraorbital optic nerve in Group 1 (n=8) exhibited a significantly lower T1-weighted signal intensity in the lateral region of the left SC compared to the left medial SC and right control SC. Partial transection toward the temporal or nasal region of the right intraorbital optic nerve in Group 2 (n=7) led to T1-weighted hypointensity in the rostral or caudal region of the left SC, whereas a clear border was observed separating 2 halves of the left SC in all groups. Previous histological and electrophysiological studies showed that the retinal ganglion cell axons emanating from superior, inferior, nasal and temporal retina projected respectively to the contralateral lateral, medial, caudal and rostral SC in rodents. While this topological pattern is preserved in the intraorbital optic nerve, it was shown that partial transection of the superior intraorbital optic nerve led to primary injury predominantly in the superior but not inferior retina and optic nerve. The results of this study demonstrated the sensitivity of submillimeter-resolution MEMRI for in vivo, 3D mapping of the precise retinotopic projections in SC upon reduced anterograde axonal transport of Mn2+ ions from localized regions of the anterior visual pathways to the subcortical midbrain nuclei. Future MEMRI studies are envisioned that measure the topographic changes in brain development, diseases, plasticity and regeneration therapies in a global and longitudinal setting. © 2010 Elsevier Inc.

Research Area(s)

  • Anterograde axonal transport, Intraorbital optic nerve, Manganese-enhanced MRI, Partial transection, Retinotopic mapping, Superior colliculus

Citation Format(s)

In vivo retinotopic mapping of superior colliculus using manganese-enhanced magnetic resonance imaging. / Chan, Kevin C.; Li, Jiang; Kau, Phillis; Zhou, Iris Y.; Cheung, Matthew M.; Lau, Condon; Yang, Jian; So, Kwok-fai; Wu, Ed X.

In: NeuroImage, Vol. 54, No. 1, 01.01.2011, p. 389-395.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review