TY - CHAP
T1 - In vivo recordings at the Caenorhabditis elegans neuromuscular junction
AU - Gao, Shangbang
AU - Hu, Zhitao
N1 - Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to [email protected].
PY - 2018
Y1 - 2018
N2 - The communication between neurons occurs via synapses, specialized connections with other cells or tissues (e.g., muscles). The function of the synapse is mainly determined by synaptic vesicle exocytosis from the presynaptic nerve terminal, which contains hundreds of vesicles that are filled with neurotransmitters. Electrophysiology provides a direct way to measure synaptic vesicle release and investigate synaptic properties. C. elegans neuromuscular junction (NMJ) has been established as a model system to study synaptic function because of the application of electrophysiological recording. Here we describe a recently developed patch-clamp recording technique in neuromuscular junction of C. elegans including details of the construction of typical electrophysiology rig, the proper dissection technique, and the recording of different types of synaptic currents. © 2018, Springer Science+Business Media, LLC.
AB - The communication between neurons occurs via synapses, specialized connections with other cells or tissues (e.g., muscles). The function of the synapse is mainly determined by synaptic vesicle exocytosis from the presynaptic nerve terminal, which contains hundreds of vesicles that are filled with neurotransmitters. Electrophysiology provides a direct way to measure synaptic vesicle release and investigate synaptic properties. C. elegans neuromuscular junction (NMJ) has been established as a model system to study synaptic function because of the application of electrophysiological recording. Here we describe a recently developed patch-clamp recording technique in neuromuscular junction of C. elegans including details of the construction of typical electrophysiology rig, the proper dissection technique, and the recording of different types of synaptic currents. © 2018, Springer Science+Business Media, LLC.
KW - C. elegans
KW - Electrophysiology
KW - Neuromuscular junction
KW - Patch clamp
KW - Synapse
KW - Synaptic vesicle
UR - https://www.scopus.com/pages/publications/85038426339
UR - https://www.scopus.com/record/pubmetrics.uri?eid=2-s2.0-85038426339&origin=recordpage
U2 - 10.1007/978-1-4939-7549-5_8
DO - 10.1007/978-1-4939-7549-5_8
M3 - RGC 12 - Chapter in an edited book (Author)
VL - 134
T3 - Neuromethods
SP - 143
EP - 156
BT - Neuromethods
PB - Humana Press Inc.
ER -