In Vivo Bioimaging of Silver Nanoparticle Dissolution in the Gut Environment of Zooplankton

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

24 Scopus Citations
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Author(s)

Detail(s)

Original languageEnglish
Pages (from-to)12212-12223
Journal / PublicationACS Nano
Volume12
Issue number12
Online published20 Nov 2018
Publication statusPublished - 26 Dec 2018
Externally publishedYes

Abstract

Release of silver ions (Ag+ ) is often regarded as the major cause for silver nanoparticle (AgNP) toxicity toward aquatic organisms. Nevertheless, differentiating AgNPs and Ag+ in a complicated biological matrix and their dissolution remains a bottleneck in our understanding of AgNP behavior in living organisms. Here, we directly visualized and quantified the time-dependent release of Ag+ from different sized AgNPs in an in vivo model zooplankton (Daphnia magna). A fluorogenic Ag+ sensor was used to selectively detect and localize the released Ag+ in daphnids. We demonstrated that the ingested AgNPs were dissoluted to Ag+ , which was heterogeneously distributed in daphnids with much higher concentration in the anterior gut. At dissolution equilibrium, a total of 8.3-9.7% of ingested AgNPs was released as Ag+ for 20 and 60 nm AgNPs. By applying a pH sensor, we further showed that the dissolution of AgNPs was partially related to the heterogeneous distribution of pH in different gut sections of daphnids. Further, Ag+ was found to cross the gills and enter the daphnids, which may be a potential pathway leading to AgNP toxicity. Our findings provided fundamental knowledge about the transformation of AgNPs and distribution of Ag+ in daphnids. ©

Research Area(s)

  • AgNPs, AIE, bioimaging, Daphnia, dissolution, gut

Citation Format(s)

In Vivo Bioimaging of Silver Nanoparticle Dissolution in the Gut Environment of Zooplankton. / Yan, Neng; Tang, Ben Zhong; Wang, Wen-Xiong.

In: ACS Nano, Vol. 12, No. 12, 26.12.2018, p. 12212-12223.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review