In vitro performance of an injectable hydrogel/microsphere based immunocyte delivery system for localised anti-tumour activity

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review

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Author(s)

  • Chunming Wang
  • Gerard N. Adrianus
  • Nan Sheng
  • Shikai Toh
  • Yihong Gong

Detail(s)

Original languageEnglish
Pages (from-to)6986-6995
Journal / PublicationBiomaterials
Volume30
Issue number36
Publication statusPublished - Dec 2009
Externally publishedYes

Abstract

The current practice of cell immunotherapy against cancer has encountered a substantial challenge, that is, targeted delivery of therapeutic cells to tumour sites is not favourably managed. In this study, we aimed to provide an engineering solution to govern the cell targeting and actions, for which a biomaterial model is developed to mediate the conveyance and accommodation of activated immunocytes with anti-cancer potentials. We fabricated a dual-layered hydrogel/microsphere (GS) composite, which preserves all advantageous features of hydrogel such as injectability and favourable permeability, to achieve genuine localisation and physical immobilisation of the executing immunocytes-macrophages. According to our presented in vitro investigations, the GS immunoconstruct exhibited effective elimination of carcinoma cells as well as high safety free of gene alteration or cell leakage. Notably, unwanted long-term proliferation of the delivered cells was restrained by physical encapsulation in the bio-inert 3D hydrogel frameworks. By these efforts, we have provided an immunocyte delivery platform with which cell-based immunotherapy can be initiated at a desired location and implemented in a controlled manner. © 2009 Elsevier Ltd. All rights reserved.

Research Area(s)

  • Apoptosis, Cancer immunotherapy, Cytokine, Hydrogel, Macrophage, Microsphere

Bibliographic Note

Publication details (e.g. title, author(s), publication statuses and dates) are captured on an “AS IS” and “AS AVAILABLE” basis at the time of record harvesting from the data source. Suggestions for further amendments or supplementary information can be sent to lbscholars@cityu.edu.hk.

Citation Format(s)

In vitro performance of an injectable hydrogel/microsphere based immunocyte delivery system for localised anti-tumour activity. / Wang, Chunming; Adrianus, Gerard N.; Sheng, Nan; Toh, Shikai; Gong, Yihong; Wang, Dong-An.

In: Biomaterials, Vol. 30, No. 36, 12.2009, p. 6986-6995.

Research output: Journal Publications and Reviews (RGC: 21, 22, 62)21_Publication in refereed journalpeer-review